Efficacy Evaluation of an Inactivated Duck Tembusu Virus Vaccine
Efficacy Evaluation of an Inactivated Duck Tembusu Virus Vaccine
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DOI：
10.1637/10960-101514-reg
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发表时间：
2015-06-01
期刊：
影响因子：
1.4
通讯作者：
Pan, Jie
中科院分区：
文献类型：
--
作者：
Lin, Jian;Liu, Yuehuan;Pan, Jie
为了评估一种基于病毒的灭活疫苗在控制和预防中国新出现的鸭天布苏病毒感染方面的潜在用途，我们在12日龄的鸭胚中繁殖了一种鸭天布苏病毒分离株Tembusu- hb，并用甲醛处理使其灭活。将灭活病毒抗原用矿物油乳化，生产出5批疫苗。对北京鸭和北京白鹅进行了免疫原性和保护效果评价。结果表明，经2次肌肉或皮下注射灭活疫苗后，80%以上的免疫鸭均能抵御强毒病毒的攻击，病毒分离结果为阴性。这种保护还与ELISA检测到的阳性病毒特异性抗体反应相关。相比之下，对照组鸭和鹅没有检测到任何抗体反应。毒株攻毒后，从所有对照鸭和鹅中分离出病毒。有趣的是，在使用同一批次疫苗接种两次的北京白鹅中观察到不同水平的保护（20%-80%），这表明疫苗具有物种特异性作用。总之，结果清楚地表明，灭活鸭天布苏病毒疫苗具有免疫原性，并对强毒病毒的攻击提供保护。
To evaluate the potential use of an inactivated virus-based vaccine for the control and prevention of the newly emerged duck Tembusu virus infection in China, a duck Tembusu virus isolate, Tembusu-HB, was propagated in 12-day-old duck embryos and inactivated by treatment with formaldehyde. The inactivated viral antigen was emulsified with mineral oil, and five batches of the vaccine were manufactured. The immunogenicity and protection efficacy of the vaccine were evaluated in Beijing ducks and Beijing white geese. Results showed that more than 80% of immunized ducks were protected against virulent virus challenge after two intramuscular or subcutaneous injections of the inactivated vaccine, as evidenced by the negative virus isolation results. The protection is also correlated with a positive virus-specific antibody response as detected by ELISA. In contrast, none of the control ducks and geese had any detectable antibody response. Virus was isolated from all control ducks and geese after virulent virus challenge. Interestingly, a variable level of protection (20%-80%) was observed in Beijing white geese immunized twice with the same batches of vaccine, suggesting a species-specific effect of the vaccine. Overall, the results clearly suggest that the inactivated duck Tembusu virus vaccine is immunogenic and provides protection against virulent virus challenge.