Comprehensive gene expression profile of a normal human liver
Comprehensive gene expression profile of a normal human liver
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DOI：
10.1006/bbrc.2000.2272
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发表时间：
2000-03-05
影响因子：
3.1
通讯作者：
Matsushima, K
中科院分区：
文献类型：
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作者：
Yamashita, T;Hashimoto, S;Matsushima, K
为了研究正常人类肝脏的基因表达谱，我们进行了基因表达序列分析(SAGE)，它允许对组织中表达的数千个基因进行定量和同时分析。从粗大的正常人肝脏标本中提取多聚腺苷化RNA，并进行SAGE。还对3个不同的正常肝脏样本分别进行了逆转录聚合酶链式反应(RT-PCR)，以评估每个个体的图谱的有效性。总共对30,982个标签进行了测序，其中8,596个标签是唯一的。在正常肝脏中高表达的基因包括编码血浆蛋白(占总转录本的21.8%)、胞浆蛋白(占8.6%)、酶(占4.8%)、蛋白酶抑制物(占1.7%)、补体(占1.1%)和凝血因子(占0.75%)。到目前为止，约有13.9%的转录本编码了GenBank中未报道的基因。这项研究确定了与各种人类肝病相关的候选基因，包括病毒性肝炎、肝硬变和肝细胞癌。(C)2000年学术出版社。
To investigate the gene expression profile of a normal human liver, we performed serial analysis of gene expression (SAGE), which allows the quantitative and simultaneous analysis of thousands of genes expressed in tissue. Polyadenylated RNA was obtained from a hulk normal human liver sample and SAGE was performed. Reverse transcriptase-polymerase chain reaction (RT-PCR) was also performed in each of 3 different normal liver samples to evaluate the validity of the profile in each individual. A total of 30,982 tags were sequenced, 8,596 of which were unique. The genes highly expressed in the normal Liver were those encoding plasma proteins (>21.8% of total transcripts), cytoplasmic proteins (>8.6%), enzymes (>4.8%), protease inhibitors (>1.7%), complements (>1.1%), and coagulation factors (>0.75%). About 13.9% of all transcripts encoded genes not reported in GenBank thus far. This study identifies candidate genes to be examined in relation to various human liver diseases, including viral hepatitis, liver cirrhosis, and hepatocellular carcinoma. (C) 2000 Academic Press.