Cholera in Lima, Peru, correlates with prior isolation of Vibrio cholerae from the environment
Cholera in Lima, Peru, correlates with prior isolation of Vibrio cholerae from the environment
复制标题
DOI：
10.1093/oxfordjournals.aje.a009235
复制
发表时间：
1997-12-15
影响因子：
5
通讯作者：
Morris, JG
中科院分区：
文献类型：
--
作者：
Franco, AA;Fix, AD;Morris, JG
从1993年11月至1995年3月，作者利用最近发展起来的DNA探针技术，对从秘鲁利马12个环境点每月采集的样品中霍乱弧菌的发生情况进行了定量数据。霍乱弧菌的峰值计数范围为10（2）/ml至10（5）/ml，在污水污染区和灌溉水中的计数最高。在7月至10月的冬季，在任何地点都发现了霍乱病例。在社区发病前，环境中可检出霍乱弧菌，上游较清洁点的霍乱弧菌数与社区发病2、3个月后的霍乱弧菌数有显著相关性。在污水污染较重的地方，社区发病前仍可检出霍乱弧菌;然而，与上游地点相反，后者的计数与同时发生的霍乱病例数关系最密切，这些数据支持霍乱季节性模型，即环境中霍乱弧菌数量的初始增加（可能由温度引发）之后是社区中的疾病发作，随着流行病循环的进行，这些人类病例进一步放大了有机体。
The authors utilized a recently developed DNA probe technique to obtain quantitative data on occurrence of Vibrio cholerae in samples collected monthly from 12 environmental sites in Lima, Peru, from November 1993 through March 1995, Peak V. cholerae counts ranged from 10(2)/ml to 10(5)/ml, with the highest counts in sewage-contaminated areas and irrigation water, With our methodology, no V, cholerae cases were detected at any site during the winter months of July through October. Counts were detectable in the environment before onset of cholera in the community, with counts at "cleaner" sites upriver correlating significantly with occurrence of community disease 2 and 3 months later, In sites with heavy sewage contamination, V. cholerae could still be detected before the onset of cases in the community; however, in contrast to upriver sites, counts at these latter sites correlated most closely with the number of concurrently occurring cholera cases, These data support a model of cholera seasonality in which initial increases in number of V, cholerae in the environment (possibly triggered by temperature) are followed by onset of illness in the community, with these human cases further amplifying the organism as the epidemic cycle proceeds.