Arabidopsis TONNEAU1 proteins are essential for preprophase band formation and interact with centrin
Arabidopsis TONNEAU1 proteins are essential for preprophase band formation and interact with centrin
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DOI：
10.1105/tpc.107.056812
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发表时间：
2008-08-01
期刊：
影响因子：
11.6
通讯作者：
Bouchez, David
中科院分区：
文献类型：
--
作者：
Azimzadeh, Juliette;Nacry, Philippe;Bouchez, David
植物细胞具有参与细胞分裂和伸长的特殊微管结构。拟南芥的tonneau 1（ton 1）突变体在形态发生、分裂平面的定位和细胞组织方面显示出严重的缺陷。这些主要是由皮质细胞骨架功能障碍和微管前期带缺失引起的。ton 1插入突变体的表征揭示了复杂的染色体重排，导致同时破坏两个高度相似的基因串联，TON 1a和TON 1b。TON 1蛋白在陆地植物中是保守的，并且与人类中心体蛋白共享序列基序。TON 1蛋白与拟南芥细胞的可溶性和微粒体组分相关联，并且绿色荧光蛋白-TON 1融合标记皮质细胞骨架结构，包括preprophase带和间期皮质阵列。酵母双杂交筛选确定拟南芥中心蛋白作为一个潜在的TON 1的合作伙伴。这种相互作用在体外和植物细胞中都得到了证实。TON 1与中心体蛋白的相似性及其与微管组织中心的另一个关键成分中心蛋白的相互作用表明，中心体参与微管阵列组织的功能在植物和动物之间的进化分歧中是保守的。
Plant cells have specific microtubule structures involved in cell division and elongation. The tonneau1 (ton1) mutant of Arabidopsis thaliana displays drastic defects in morphogenesis, positioning of division planes, and cellular organization. These are primarily caused by dysfunction of the cortical cytoskeleton and absence of the preprophase band of microtubules. Characterization of the ton1 insertional mutant reveals complex chromosomal rearrangements leading to simultaneous disruption of two highly similar genes in tandem, TON1a and TON1b. TON1 proteins are conserved in land plants and share sequence motifs with human centrosomal proteins. The TON1 protein associates with soluble and microsomal fractions of Arabidopsis cells, and a green fluorescent protein-TON1 fusion labels cortical cytoskeletal structures, including the preprophase band and the interphase cortical array. A yeast two-hybrid screen identified Arabidopsis centrin as a potential TON1 partner. This interaction was confirmed both in vitro and in plant cells. The similarity of TON1 with centrosomal proteins and its interaction with centrin, another key component of microtubule organizing centers, suggests that functions involved in the organization of microtubule arrays by the centrosome were conserved across the evolutionary divergence between plants and animals.