Gonadotropin-induced loss of hormone receptors and desensitization of adenylate cyclase in the ovary.
Gonadotropin-induced loss of hormone receptors and desensitization of adenylate cyclase in the ovary.
复制标题
促性腺激素诱导卵巢中激素受体的丧失和腺苷酸环化酶的脱敏。
DOI：
10.1016/s0021-9258(17)32914-9
复制
发表时间：
1976
期刊：
影响因子：
--
通讯作者：
K. Catt
中科院分区：
文献类型：
--
作者：
M. Conti;J. P. Harwood;A. Hsueh;M. Dufau;K. Catt
对注射人绒毛膜促性腺激素(HCG)的黄体大鼠卵巢中促性腺激素受体位置和腺苷环化酶活性进行了分析。促性腺激素结合量和腺苷环化酶激素刺激在6~12h迅速下降至最低，持续抑制4d，5~7d恢复至对照水平。在氟作用下，腺苷环化酶总活性在几小时内下降50%，但在24小时后恢复正常，促性腺激素受体数目与腺苷环化酶受激素刺激的能力密切相关。组织结合激素测定表明，最初激素敏感性和结合能力的丧失与黄体生成素受体位置的占用有关，但这些活性的长期变化与受体占用无关。这些研究表明，促性腺激素诱导卵巢腺苷环化酶处于难治或不敏感状态是由于丢失了特定的激素受体位点。
Gonadotropin receptor sites and adenylate cyclase activity were analyzed in luteinized rat ovaries following injection of human chorionic gonadotropin (hCG). Gonadotropin binding capacity and hormonal stimulation of adenylate cyclase declined rapidly to a minimum at 6 to 12 h, remained depressed for 4 days, and returned to the control level between 5 and 7 days. Total adenylate cyclase activity measured in the presence of fluoride fell by 50% within a few hours but returned to normal by 24 h. A close correlation was observed between the number of gonadotropin receptors and the ability of adenylate cyclase to be stimulated by hormone. Assay of tissue-bound hormone showed that the initial loss of hormone sensitivity and binding capacity was associated with occupancy of luteinizing hormone receptor sites, but that the prolonged changes in these activities were not attributable to receptor occupancy. These studies have demonstrated that induction of a refractory or desensitized state in ovarian adenylate cyclase by gonadotropin results from the loss of specific hormone receptor sites.