Positioning and stability of nucleosomes on MMTV 3′LTR sequences
Positioning and stability of nucleosomes on MMTV 3′LTR sequences
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DOI：
10.1006/jmbi.1997.1464
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发表时间：
1998-01-23
影响因子：
5.6
通讯作者：
Richmond, TJ
中科院分区：
文献类型：
--
作者：
Flaus, A;Richmond, TJ
在体外以碱基对分辨率将唯一定位的核小体定位在小鼠乳腺肿瘤3'长末端重复序列（MMTV 3'LTR）DNA上。核小体A的组装强烈优于核小体B，并且每个核小体作为单体的加热导致以独特的速率迁移到DNA片段的末端。与DNA序列分析一起，这表明了为什么MMTV 3'LTV核小体位置报告的载体衍生序列上游冲突，以及侧翼基因组序列如何在体内情况下调节启动子。重要的是，核小体显示出在生理相关条件下沿着DNA迁移显著的距离沿着，并且已经在溶液中直接测量了实际速率。精确定位和移位超过60 bp具有重要的后果，转录因子访问该MMTV启动子和核小体的作用一般。(C)出版社：Academic Press Limited。
Uniquely positioned nucleosomes were mapped in vitro on mouse mammary tumor 3' long terminal repeat (MMTV 3'LTR) DNA at base-pair resolution. Nucleosome A assembly was strongly favored over nucleosome B, and heating of each as a mononucleosome caused migration to the ends of the DNA fragment at a unique rate. Taken together with DNA sequence analysis, this suggests why MMTV 3'LTV nucleosome positions reported upstream of vector-derived sequences conflict and also how flanking genomic sequences could modulate the promoter in in vivo situations. Importantly, nucleosomes are shown to migrate for significant distances along DNA under physiologically relevant conditions, and the actual rates have been measured directly in solution. Exact positioning and shifting over greater than 60 bp has important consequences for transcription factor access to this MMTV promoter and for the role of nucleosomes in general. (C) 1998 Academic Press Limited.