Intraplantar injection of bergamot essential oil induces peripheral antinociception mediated by opioid mechanism
Intraplantar injection of bergamot essential oil induces peripheral antinociception mediated by opioid mechanism
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DOI：
10.1016/j.pbb.2010.09.020
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发表时间：
2011-01-01
影响因子：
3.6
通讯作者：
Sakurada, Shinobu
中科院分区：
文献类型：
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作者：
Sakurada, Tsukasa;Mizoguchi, Hirokazu;Sakurada, Shinobu
本研究调查了含有芳樟醇和乙酸芳樟酯作为主要挥发性成分的佛手柑精油（BEO）在辣椒素测试中的效果。足底内注射辣椒素（1.6μg）对注射的爪子产生短暂的舔/咬反应。足底注射 BEO 可剂量依赖性地抑制辣椒素引起的伤害性行为反应。注射到后爪的芳樟醇和乙酸芳樟酯均显示出伤害性反应显着降低，这比 BEO 更有效。使用盐酸纳洛酮（一种阿片受体拮抗剂）进行腹膜内 (i.p.) 和足底内预处理，可显着逆转 BEO 和芳樟醇诱导的镇痛作用。用甲碘纳洛酮（一种外周作用的 mu-阿片受体偏好拮抗剂）进行预处理，对 BEO 和芳樟醇诱导的镇痛产生显着的拮抗作用。腹膜内注射引起的抗伤害作用或联合注射 BEO 或芳樟醇可增强鞘内吗啡的作用。 BEO或芳樟醇与吗啡组合的增强作用被盐酸纳洛酮预处理所拮抗。我们的结果为外周阿片类药物参与 BEO 和芳樟醇诱导的镇痛作用提供了证据。作用于外周部位的 BEO 或芳樟醇与吗啡联合给药可能是治疗临床疼痛的一种有前途的方法。 (C) 2010 Elsevier Inc. 保留所有权利。
This study investigated the effect of bergamot essential oil (BEO) containing linalool and linalyl acetate as major volatile components in the capsaicin test. The intraplantar injection of capsaicin (1.6 mu g) produced a short-lived licking/biting response toward the injected paw. The nociceptive behavioral response evoked by capsaicin was inhibited dose-dependently by intraplantar injection of BEO. Both linalool and linalyl acetate, injected into the hindpaw, showed a significant reduction of nociceptive response, which was much more potent than BEO. Intraperitoneal (i.p.) and intraplantar pretreatment with naloxone hydrochloride, an opioid receptor antagonist, significantly reversed BEO- and linalool-induced antinociception. Pretreatment with naloxone methiodide, a peripherally acting mu-opioid receptor preferring antagonist, resulted in a significant antagonizing effect on antinociception induced by BEO and linalool. Antinociception induced by i.p. or intrathecal morphine was enhanced by the combined injection of BEO or linalool. The enhanced effect of combination of BEO or linalool with morphine was antagonized by pretreatment with naloxone hydrochloride. Our results provide evidence for the involvement of peripheral opioids, in the antinociception induced by BEO and linalool. Combined administration of BEO or linalool acting at the peripheral site, and morphine may be a promising approach in the treatment of clinical pain. (C) 2010 Elsevier Inc. All rights reserved.