EFFECTS OF SOMAN-INDUCED SEIZURES ON DIFFERENT EXTRACELLULAR AMINO-ACID LEVELS AND ON GLUTAMATE UPTAKE IN RAT HIPPOCAMPUS
EFFECTS OF SOMAN-INDUCED SEIZURES ON DIFFERENT EXTRACELLULAR AMINO-ACID LEVELS AND ON GLUTAMATE UPTAKE IN RAT HIPPOCAMPUS
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DOI：
10.1016/0006-8993(91)91539-d
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发表时间：
1991-11-01
期刊：
影响因子：
2.9
通讯作者：
BLANCHET, G
中科院分区：
文献类型：
--
作者：
LALLEMENT, G;CARPENTIER, P;BLANCHET, G
用颅内微透析法测定了乙酰胆碱酯酶抑制剂梭曼（0- 1，2，2-trimethylpropylmethylphosphonofluoridate）诱发大鼠癫痫发作过程中海马CA 3和CA 1区细胞外氨基酸的含量。在另一系列动物的海马匀浆中测定谷氨酸摄取水平。癫痫发作30分钟内，CA 3细胞外谷氨酸水平出现早期和短暂的增加，牛磺酸、甘氨酸和谷氨酰胺水平出现相关的短暂升高。谷氨酸水平在CA 1早期增加，下降，然后变得更加持续（癫痫发作50分钟后）。在CA 1的牛磺酸，甘氨酸和谷氨酰胺水平明显升高伴随着谷氨酸浓度的变化。谷氨酸水平的变化与谷氨酸摄取的增加，迅速下降后40分钟的癫痫发作。被认为是短暂释放谷氨酸在CA 3和持续释放CA 1在长期梭曼诱导的癫痫发作的作用。研究了谷氨酸与其它氨基酸释放的相关性。
Extracellular amino acid levels in CA3 and CA1 fields of rat hippocampus, an area highly sensitive to seizures, were determined by intracranial microdialysis during seizures induced by systemic administration of soman (0-1,2,2-trimethylpropyl methylphosphonofluoridate), a potent inhibitor of acetylcholinesterase. The glutamate uptake level was determined on another series of animals in hippocampus homogenates. An early and transient increase in the extracellular glutamate level occurred in CA3 within 30 min of seizures, with correlated brief elevations of taurine, glycine and glutamine levels. The glutamate level increased early in CA1, declined and then became more sustained (after 50 min of seizures). Apparent elevations of taurine, glycine and glutamine levels in CA1 accompanied changes in glutamate concentrations. Changes of glutamate level correlated with an increase in the glutamate uptake which rapidly declined after 40 min of seizures. The role of the transient release of glutamate in CA3 and of the sustained release in CA1 in prolonged soman-induced seizures is considered. The correlation between glutamate and other amino acid release is studied.