Micro-computed tomography with iodine staining resolves the arrangement of muscle fibres
Micro-computed tomography with iodine staining resolves the arrangement of muscle fibres
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DOI：
10.1016/j.jbiomech.2010.08.027
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发表时间：
2011-01-04
影响因子：
2.4
通讯作者：
Cox, Philip G.
中科院分区：
文献类型：
--
作者：
Jeffery, Nathan S.;Stephenson, Robert S.;Cox, Philip G.
我们在这里举例说明microCT图像，其中肌肉和结缔组织之间的对比度已经通过碘染色实现。增强被证明是依赖于碘溶液（I2KI）的浓度，在溶液中的时间和试样的大小。组织学检查证实，在增强的microCT图像上可以看到单个肌纤维的排列，并且碘优先于周围的结缔组织在肌纤维中积累。我们探讨应用这种技术来描述骨骼肌的纤维结构，并得出结论，它有可能成为一种非破坏性和成本效益的方法，调查肌束结构，特别是在比较形态学研究。(C)2010爱思唯尔有限公司版权所有。
We illustrate here microCT images in which contrast between muscle and connective tissue has been achieved by means of staining with iodine. Enhancement is shown to be dependent on the concentration of iodine solution (I2KI), time in solution and specimen size. Histological examination confirms that the arrangement of individual muscle fibres can be visualised on the enhanced microCT images, and that the iodine accumulates in the muscle fibres in preference to the surrounding connective tissues. We explore the application of this technique to describe the fibrous structure of skeletal muscle, and conclude that it has the potential to become a non-destructive and cost-effective method for investigating muscle fascicle architecture, particularly in comparative morphological studies. (C) 2010 Elsevier Ltd. All rights reserved.