Evaluation of the leucine incorporation technique for detection of pollution-induced community tolerance to copper in a long-term agricultural field trial with urban waste fertilizers
Evaluation of the leucine incorporation technique for detection of pollution-induced community tolerance to copper in a long-term agricultural field trial with urban waste fertilizers
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DOI：
10.1016/j.envpol.2014.07.013
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发表时间：
2014-11-01
影响因子：
8.9
通讯作者：
Brandt, Kristian Koefoed
中科院分区：
文献类型：
--
作者：
Lekfeldt, Jonas Duus Stevens;Magid, Jakob;Brandt, Kristian Koefoed
众所周知，铜（Cu）在接受城市废物作为肥料的农业土壤中积累。我们在此报告了在长期的农业田间试验中使用亮氨酸掺入技术来确定污染诱导的社区对铜的耐受性（Leu-PICT）。有机肥改良土壤细菌群落对铜的耐受性显著提高，且与土壤全铜量呈正相关。然而，金属形态形成和全细胞细菌生物传感器分析表明，在Leu-PICT检测过程中，观察到的PICT反应可以完全由Cu形态形成和生物利用度伪像来解释。因此，模拟使用超过100年的城市废物（污水污泥或堆肥城市废物）的农业应用并没有产生足够的铜积累来选择铜抗性。我们的发现也对以前发表的PICT现场研究有启示，并表明需要严格的PICT检测标准来现场识别特定的毒物。(C) 2014 Elsevier Ltd.版权所有。
Copper (Cu) is known to accumulate in agricultural soils receiving urban waste products as fertilizers. We here report the use of the leucine incorporation technique to determine pollution-induced community tolerance (Leu-PICT) to Cu in a long-term agricultural field trial. A significantly increased bacterial community tolerance to Cu was observed for soils amended with organic waste fertilizers and was positively correlated with total soil Cu. However, metal speciation and whole-cell bacterial biosensor analysis demonstrated that the observed PICT responses could be explained entirely by Cu speciation and bioavailability artifacts during Leu-PICT detection. Hence, the agricultural application of urban wastes (sewage sludge or composted municipal waste) simulating more than 100 years of use did not result in sufficient accumulation of Cu to select for Cu resistance. Our findings also have implications for previously published PICT field studies and demonstrate that stringent PICT detection criteria are needed for field identification of specific toxicants. (C) 2014 Elsevier Ltd. All rights reserved.