GENERATION OF REASSORTANTS BETWEEN AFRICAN ARENAVIRUSES
GENERATION OF REASSORTANTS BETWEEN AFRICAN ARENAVIRUSES
复制标题
DOI：
10.1016/0042-6822(92)90514-p
复制
发表时间：
1992-06-01
期刊：
影响因子：
3.7
通讯作者：
LUKASHEVICH, IS
中科院分区：
文献类型：
--
作者：
LUKASHEVICH, IS
拉沙病毒 (LAS) 和莫佩亚病毒 (MOP) 是非洲沙粒病毒，由野生啮齿动物携带，偶尔会传染给人类。在人类和非人类灵长类动物中，拉沙病毒会导致 60% 未经治疗的病例死亡，而 Mopeia 不会导致死亡，并且已知可以保护猴子免受拉沙病毒的致命攻击。这两种非洲沙粒病毒对乳交系小鼠的致死率以及琼脂覆盖下的斑块大小也有所不同。 MOP 病毒脑内 (ic) 接种后会引起小斑块和致命感染。相比之下，LAS 接种不会杀死小鼠，并且病毒会诱发大斑块。用 LAS 和 MOP 病毒共感染 Vero 细胞后，分离并纯化了一些产生小噬斑且对远交小鼠不致死的表型重配体。使用特异于 L 和 S RNA 片段的 LAS 和 MOP cDNA 探针进行斑点印迹杂交，揭示了由 MOP 的 L RNA 和 LAS 的 S RNA（MOP/LAS 重配体）组成的基因型。过继转移实验证明，腹膜内感染 MOP/LAS 重配体的 CBA 小鼠的免疫脾细胞在接种 LAS 病毒后能够保护受体小鼠免受致命疾病的影响。
Lassa (LAS) and Mopeia (MOP) viruses are African arenaviruses which are carried by wild rodents and occasionally transferred to humans. In humans and nonhuman primates, Lassa causes mortality in 60% of untreated cases, whereas Mopeia does not cause mortality and has been known to protect monkeys from lethal challenge with Lassa. These two African arenaviruses also differ in their lethality for suckling outbred mice and in their plaque sizes under agar overlay. MOP virus induces small plaques and lethal infection after intracerebral (ic) inoculation. In contrast, LAS inoculation does not kill mice and the virus induces large plaques. After coinfection of Vero cells with LAS and MOP viruses some phenotypic reassortants which produced small plaques and were not lethal for outbred mice were isolated and plaque-purified. Dot-blot hybridization using LAS and MOP cDNA probes specific for L and S RNA segments revealed a genotype consisting of the L RNA of MOP and the S RNA of LAS (MOP/LAS reassortant). Adoptive transfer experiments demonstrated an ability of immune splenocytes from CBA mice intraperitoneally infected with the MOP/LAS reassortants to protect recipient mice against lethal disease after is inoculation with LAS virus.