OVEREXPRESSION OF VINCULIN SUPPRESSES CELL MOTILITY IN BALB/C 3T3 CELLS
OVEREXPRESSION OF VINCULIN SUPPRESSES CELL MOTILITY IN BALB/C 3T3 CELLS
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DOI：
10.1002/cm.970220206
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发表时间：
1992-01-01
影响因子：
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通讯作者：
BENZEEV, A
中科院分区：
文献类型：
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作者：
FERNANDEZ, JLR;GEIGER, B;BENZEEV, A
在BALB/c 3T3细胞中，通过基因转染法，细胞内局灶性接触和细胞-细胞连接中的胞浆蛋白纽蛋白含量增加。从鸡全长cDNA中表达的纽蛋白掺入焦点接触蛋白中，其模式与内源蛋白相同。在内源性水平上稳定表达纽蛋白20%的细胞已经改变了运动特性。在这些细胞中，向融合单层形成的伤口中迁移的能力和单个细胞的运动能力显著降低。这些结果提供了直接证据，表明细胞运动可以通过调节纽蛋白的表达来调节。
The content of vinculin, a cytoplasmic protein found in focal contacts and cell-cell junctions, was increased in BALB/c 3T3 cells by gene transfection. The vinculin expressed from the full length chicken cDNA, incorporated into focal contacts and its pattern was identical to that of the endogenous protein. Cells stably expressing vinculin by 20% over the endogenous level had altered locomotory properties. In these cells, the ability to migrate into a wound formed in a confluent monolayer and the locomotion of individual cells were drastically reduced. The results provide direct evidence that cell locomotion can be regulated by modulating vinculin expression.