Serum Metabolic Signatures Induced By a Three-Day Intensified Exercise Period Persist After 14 h of Recovery in Runners
Serum Metabolic Signatures Induced By a Three-Day Intensified Exercise Period Persist After 14 h of Recovery in Runners
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DOI：
10.1021/pr400717j
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发表时间：
2013-10-01
影响因子：
4.4
通讯作者：
Lila, Mary Ann
中科院分区：
文献类型：
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作者：
Nieman, David C.;Shanely, R. Andrew;Lila, Mary Ann
这项研究调查了3天强化训练引起的人体血清代谢组的变化。跑步者(N=15，平均+/-SD年龄，35.2+/-8.7岁)在跑步机上以相当于70%最大摄氧量的速度跑2.5h/d，连续3d，分别在运动前、运动后即刻和运动后14h采集血样。样品用气相色谱和液质联用(GC-MS，LC-MS)进行分析，通过与2800多个纯化标准进行比较，确定了化合物。重复测量的ANOVA被用来识别随时间显著不同的代谢物，多项测试通过错误发现率(FDR)(Q值)进行修正。在为期3天的运动期结束后，立即测量到75种代谢物显著增加了2倍或更多，除了22种与脂肪/肉碱代谢有关的代谢物，13种与氨基酸/多肽代谢有关的代谢物，4种与血红蛋白/卟啉代谢有关的代谢物，3种与克雷布斯循环中间产物有关的代谢物(Q值和lt；0.001)。经过14小时的夜间恢复期，75种代谢物中有50种仍然升高，8种降低(主要是与氨基酸有关的代谢物)(Q值和lt；0.05)。在前20种代谢物中，运动后即刻和14h的平均倍数变化分别为12.4+/-5.3和2.9+/-1.3。运动后22种代谢物(主要与溶脂和胆汁酸代谢有关)显著下降(40-70%，Q<；0.025)，休息14小时后，除4种代谢物外，其余仍下降(Q<；0.01)。跑步者在3天的大负荷运动后，血液中与能量产生相关的代谢产物发生了深刻的系统性变化，特别是从脂质超途径，但在14小时恢复后仍未完全恢复到运动前的水平。
This study investigated changes in the human serum metabolome elicited by a 3-day period of intensified training. Runners (N = 15, mean +/- SD age, 35.2 +/- 8.7 years) ran for 2.5 h/day on treadmills at similar to 70% VO2max, for 3 days in a row, with blood samples collected pre-exercise, and immediately and 14 h post-exercise. Samples were analyzed using gas and liquid chromatography/mass spectrometry (GC-MS, LC-MS), with compounds identified based on comparison to more than 2800 purified standards. Repeated measures ANOVA was used to identify metabolites that differed significantly across time, with multiple testing corrected by the false discovery rate (FDR) (q-value). Immediately following the 3-day exercise period, significant 2-fold or higher increases in 75 metabolites were measured, with all but 22 of these metabolites related to lipid/carnitine metabolism, 13 to amino acid/peptide metabolism, 4 to hemoglobin/porphyrin metabolism, and 3 to Krebs cycle intermediates (q-values < 0.001). After a 14 h overnight recovery period, 50 of the 75 metabolites remained elevated, with 8 decreased (primarily amino acid-related metabolites) (q-values < 0.05). Among the top 20 metabolites, the mean fold changes were 12.4 +/- 5.3 and 2.9 +/- 1.3 immediately and 14-h post-exercise, respectively. Significant decreases (40-70%, q < 0.01) in 22 metabolites (primarily related to lysolipid and bile acid metabolism) were measured post-exercise, with all but 4 of these still decreased after 14 h rest recovery (q < 0.025). Runners experienced a profound systemic shift in blood metabolites related to energy production especially from the lipid super pathway following 3 days of heavy exertion that was not fully restored to pre-exercise levels after 14 h recovery.