A toolbox for validation of mass spectrometry peptides identification and generation of database: IRMa
A toolbox for validation of mass spectrometry peptides identification and generation of database: IRMa
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DOI：
10.1093/bioinformatics/btp301
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发表时间：
2009-08-01
期刊：
影响因子：
5.8
通讯作者：
Bruley, Christophe
中科院分区：
文献类型：
--
作者：
Dupierris, Veronique;Masselon, Christophe;Bruley, Christophe
IRMa工具箱提供了一个交互式应用程序，以协助验证Mascot（R）搜索结果。它允许自动。筛选Mascot（R）鉴定结果以及手动确认或拒绝单个PSM（碎片质谱和肽之间的匹配）。动态分组和信息的一致性由软件在真实的时间内保持。验证结果可以以各种形式导出，包括标识数据库（MSIdb）。这使得生物学家可以在一个独特的存储库中编译整个研究的搜索结果，以提供其项目的摘要视图。IRMa还具有完全自动化的版本，可用于高吞吐量管道。给定过滤器参数，它可以删除没有显著PSM的命中，重组由相同肽识别的命中，并将结果导出为指定格式，而无需用户干预。
The IRMa toolbox provides an interactive application to assist in the validation of Mascot (R) search results. It allows automatic. ltering of Mascot (R) identification results as well as manual confirmation or rejection of individual PSM (a match between a fragmentation mass spectrum and a peptide). Dynamic grouping and coherence of information are maintained by the software in real time. Validated results can be exported under various forms, including an identification database (MSIdb). This allows biologists to compile search results from a whole study in a unique repository in order to provide a summarized view of their project. IRMa also features a fully automated version that can be used in a high-throughput pipeline. Given filter parameters, it can delete hits with no significant PSM, regroup hits identified by the same peptide(s) and export the result to the specified format without user intervention.