Karyotype and chromosome location of characteristic tandem repeats in the pufferfish Tetraodon nigroviridis
Karyotype and chromosome location of characteristic tandem repeats in the pufferfish Tetraodon nigroviridis
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DOI：
10.1159/000015484
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发表时间：
2000-01-01
期刊：
影响因子：
--
通讯作者：
Bernot, A
中科院分区：
文献类型：
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作者：
Fischer, C;Ozouf-Costaz, C;Bernot, A
Tetraodon nigroviridis 是一种属于 Tetraodontidae 科的河豚，具有小型紧凑基因组 (385 Mb)，目前我们实验室正在对其进行核型分析，表明该物种有 2n = 42 条染色体。较小的染色体尺寸（最大的一对测量值小于 3 μm）使得仅基于形态的精确染色体配对变得复杂。然而，DAPI 染色提供了条带状图案。由于某些异染色质类别的数量变化，无法精确建立染色体公式，但估计包括大约 20 条间位或亚中着丝粒染色体和 22 条亚端着丝粒染色体。 FISH 已明确定位着丝粒卫星、端粒重复序列以及主要和次要 rRNA 簇。因此，28S和5S rDNA序列可用作染色体特异性探针。版权所有 (C) 2000 S. Karger AG，巴塞尔。
Karyotype analysis of Tetraodon nigroviridis, a pufferfish of the family Tetraodontidae with a small compact genome (385 Mb) which is currently being investigated in our laboratory, indicates that this species has 2n = 42 chromosomes. The small chromosome size (the largest pair measuring less than 3 mu m) has complicated accurate chromosome pairing based on morphology alone. DAPI staining, however, provides a banding-like pattern. Because of quantitative variations of some heterochromatin classes, the chromosome formula can not be established precisely, but is estimated to include approximately 20 meta- or submetacentric chromosomes and 22 subtelocentric chromosomes. A centromeric satellite, telomeric repeats, and the major and minor rRNA clusters have been localized unequivocally by FISH. As a result, the 28S and 5S rDNA sequences can be used as chromosome-specific probes. Copyright (C) 2000 S. Karger AG, Basel.