Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black Spot.
Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black Spot.
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DOI：
10.3767/003158511x569169
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发表时间：
2011-06
期刊：
影响因子：
9.1
通讯作者：
Crous PW
中科院分区：
文献类型：
--
作者：
Glienke C;Pereira OL;Stringari D;Fabris J;Kava-Cordeiro V;Galli-Terasawa L;Cunnington J;Shivas RG;Groenewald JZ;Crous PW
我们调查的身份和遗传多样性的100多个菌株属于Phyllosticta（有性型Guignardia），特别强调Phyllosticta citricarpa和Guignardia mangiferae s.l.发生在柑橘上。柑橘黑斑病是柑橘黑斑病的病原体，受欧盟植物检疫法规的约束。本种经常与一个分类群混淆，通常称为G。mangiferae是Capitalensis的假定有性型，Capitalensis是一种非致病性内生菌，通常分离自柑橘叶和果实以及广泛的其他宿主。nrDNA内转录间隔区（ITS 1，5.8S nrDNA，ITS 2）和部分翻译延伸因子1-α（TEF 1），肌动蛋白和甘油醛-3-磷酸脱氢酶（GPDH）基因的DNA序列分析解决了9个分支相关的7个已知的，和两个显然未描述的物种。巴西柑桔叶点霉（Phyllosticta citribraziliensis）是巴西柑桔上的一种新的内生真菌。在澳大利亚新采集的材料中，将P. citricarpa命名为一个表型，它不同于目前只在C.来自亚洲的Maxima。本文报道了巴西兰科植物Bifrenaria harrisariae上的一种新的叶点霉（Phyllosticta bifrenariae）。它在形态上不同于P. capitalensis，后者最初在巴西的Stanhopea（兰科）中描述;这里指定了一个表型。芒果球座腔菌（Guignardia mangiferae）最初描述于印度的芒果（漆树科（Anacardiaceae）），其与非致病性内生菌巴西球座腔菌（P. brazilianiae sp. nov.）这在M上是常见的。印度在巴西。此外，一个组合的系统发育树显示，P. capitalensis s.l.进化枝与G.芒果。这个分支有几个名称，最古老的是P. capitalensis。这些结果表明，在广泛宿主范围内出现的内生非致病性分离株更正确地称为首都青霉。然而，更多的基因需要进行分析，以充分解决仍然观察到的形态变化在这个分支。
We investigated the identity and genetic diversity of more than 100 isolates belonging to Phyllosticta (teleomorph Guignardia), with particular emphasis on Phyllosticta citricarpa and Guignardia mangiferae s.l. occurring on Citrus. Phyllosticta citricarpa is the causal agent of Citrus Black Spot and is subject to phytosanitary legislation in the EU. This species is frequently confused with a taxon generally referred to as G. mangiferae, the presumed teleomorph of P. capitalensis, which is a non-pathogenic endophyte, commonly isolated from citrus leaves and fruits and a wide range of other hosts. DNA sequence analysis of the nrDNA internal transcribed spacer region (ITS1, 5.8S nrDNA, ITS2) and partial translation elongation factor 1-alpha (TEF1), actin and glyceraldehyde-3-phosphate dehydrogenase (GPDH) genes resolved nine clades correlating to seven known, and two apparently undescribed species. Phyllosticta citribraziliensis is newly described as an endophytic species occurring on Citrus in Brazil. An epitype is designated for P. citricarpa from material newly collected in Australia, which is distinct from P. citriasiana, presently only known on C. maxima from Asia. Phyllosticta bifrenariae is newly described for a species causing leaf and bulb spots on Bifrenaria harrisoniae (Orchidaceae) in Brazil. It is morphologically distinct from P. capitalensis, which was originally described from Stanhopea (Orchidaceae) in Brazil; an epitype is designated here. Guignardia mangiferae, which was originally described from Mangifera indica (Anacardiaceae) in India, is distinguished from the non-pathogenic endophyte, P. brazilianiae sp. nov., which is common on M. indica in Brazil. Furthermore, a combined phylogenetic tree revealed the P. capitalensis s.l. clade to be genetically distinct from the reference isolate of G. mangiferae. Several names are available for this clade, the oldest being P. capitalensis. These results suggest that endophytic, non-pathogenic isolates occurring on a wide host range would be more correctly referred to as P. capitalensis. However, more genes need to be analysed to fully resolve the morphological variation still observed within this clade.