Comparison of osteoblast responses to hydroxyapatite and hydroxyapatite/soluble calcium phosphate composites
Comparison of osteoblast responses to hydroxyapatite and hydroxyapatite/soluble calcium phosphate composites
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DOI：
10.1002/jbm.a.30146
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发表时间：
2005-02-01
影响因子：
4.9
通讯作者：
Umakoshi, Y
中科院分区：
文献类型：
--
作者：
Ogata, K;Imazato, S;Umakoshi, Y
羟基磷灰石/可溶性磷酸钙复合材料（HAp/SCaP）是一种新型的羟基磷灰石基材料，它是通过真空退火制备的。本研究使用MC 3 T3-E1细胞培养系统比较了HAp和HAp/SCaP对成骨细胞增殖、分化和矿化的影响。将MC 3 T3-E1细胞培养在HAp或HAp/SCaP上，并检查贴壁细胞的数量和它们的形态。HAp/SCaP提取物对成骨细胞分化的影响通过碱性磷酸酶活性的测量和成骨细胞标志物表达的逆转录-聚合酶链反应分析来确定。此外，通过用茜素红染色钙沉积物来评价矿化。在孵育7天或更长时间后，与HAp相比，在HAp/SCaP上观察到更多数量的细胞附着，这些细胞在形态上没有退化。用HAp/SCaP的提取物培养细胞导致碱性磷酸酶活性的促进、1型胶原的表达和骨样组织的形成。本研究的结果表明，HAp/SCaP通过增加细胞外基质的胶原合成和钙化而显示出比HAp更强的成骨能力。(C)2004 Wiley Periodicals，Inc.
Hydroxyapatite/soluble calcium phosphate composites (HAp/SCaP) are novel HAp-based materials with enhanced solubility that have been developed by annealing HAp in a vacuum. This study compared the effects of HAp and HAp/SCaP on osteoblast proliferation, differentiation, and mineralization using an MC3T3-E1 cell culture system. MC3T3-E1 cells were cultured on HAp or HAp/SCaP, and the number of attached cells and their morphology were examined. The influence of the extract from HAp/SCaP on osteoblast differentiation was determined by the measurement of alkaline phosphatase activity and reverse transcriptase-polymerase chain reaction analysis of the expression of osteoblastic markers. In addition, mineralization was evaluated by the staining of calcium deposits with Alizarin red. Attachment of a greater number of cells exhibiting no degeneration in their morphology was observed on HAp/SCaP compared with HAp after incubation for 7 days or more. Culturing cells with the extract from HAp/SCaP resulted in promotion of alkaline phosphatase activity, the expression of type 1 collagen, and bone-like tissue formation. The results of the present study indicate that HAp/SCaP shows greater ability in osteogenesis than HAp by increasing collagen synthesis and calcification of the extracellular matrix. (C) 2004 Wiley Periodicals, Inc.