Comparison of the Pathogenicity of Two Different Branches of Senecavirus a Strain in China
Comparison of the Pathogenicity of Two Different Branches of Senecavirus a Strain in China
复制标题
塞内卡病毒a株两个不同分支在我国的致病力比较
DOI：
10.3390/pathogens9010039
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发表时间：
2020-01-01
期刊：
影响因子：
3.7
通讯作者：
Li, Xiangmin
中科院分区：
文献类型：
--
作者：
Zhang, Huawei;Chen, Pin;Li, Xiangmin
塞内卡病毒A （SVA）是一种与猪特发性水疱病相关的新发传染病。本文研究了生长育肥猪中不同菌株SVA的发病机制。我们的目的是评估两种不同SVA菌株的复制特性、病毒颗粒形态、临床症状和水疱病变。小鼠经鼻经（3 mL, 109TCID50/mL）感染SVA HB-CH-2016或CH/AH-02/2017，接种后（dpi）每天监测14 d的临床症状和水疱病变。在血液、粪便拭子和鼻拭子样本中检测到病毒血症或病毒脱落。结果显示，SVA HB-CH-2016和CH/AH-02/2017菌株在斑块大小、复制能力和特征病毒粒子方面无显著差异。动物实验结果表明，SVA CH/AH-02/2017和SVA HB-CH-2016均可感染猪。然而，SVA HB-CH-2016与CH/AH-02/2017菌株在致病性和感染动力学方面存在明显差异。SVA CH/AH-02/2017的发病机制与已发表的美国菌株的结果相似，而SVA HB-CH-2016菌株对猪的致病性较低。观察SVA CH/ ah -02/2017感染猪的临床症状和水疱病变。此外，SVA的不同分支应该能够诱导广泛的交叉反应性中和抗体，这在清除SVA病毒中起重要作用。该研究对SVA感染动物模型的研究将有助于疫苗和抗病毒药物的开发。
Senecavirus A (SVA), an emerging infectious disease, is associated with the porcine idiopathic vesicular disease. Here, the pathogenesis of different strains of SVA was investigated in growing-finishing pigs. We aimed to evaluate the replication characteristics, virus particle morphology, clinical signs, and vesicular lesions in comparison with two different strains of SVA. The animals were infected with SVA HB-CH-2016 or CH/AH-02/2017 by intranasal routes (3 mL, 109TCID50/mL) and monitored daily for 14 days post-inoculation (dpi) for clinical signs and vesicular lesions. Viremia or viral shedding was detected in the blood, fecal swab, and nasal swab samples. Results showed no distinct differences in plaque size, replication ability, and characteristic virions between SVA HB-CH-2016 and CH/AH-02/2017 strains. Animal experimental results showed that both SVA CH/AH-02/2017 and SVA HB-CH-2016 could infect pigs. However, an obvious difference in the pathogenicity and dynamics of infection was observed between SVA HB-CH-2016 and CH/AH-02/2017 strains. The pathogenesis of SVA CH/AH-02/2017 was similar to that of published results of USA strains, whereas the SVA HB-CH-2016 strain had low pathogenicity to pigs. Clinical signs and vesicular lesions were observed in SVA CH/AH-02/2017-infected pigs. Additionally, the different branches of SVA should be capable of inducing broad cross-reactive neutralizing antibodies, which play an important role in clearing the SVA virus. This study of animal models for SVA infection will be beneficial to develop vaccines and antivirals.