Acupuncture at Both ST25 and ST37 Improves the Pain Threshold of Chronic Visceral Hypersensitivity Rats
Acupuncture at Both ST25 and ST37 Improves the Pain Threshold of Chronic Visceral Hypersensitivity Rats
复制标题
DOI：
10.1007/s11064-009-9972-1
复制
发表时间：
2009-11-01
影响因子：
4.4
通讯作者：
Wu, Huan-Gan
中科院分区：
文献类型：
--
作者：
Liu, Hui-Rong;Wang, Xiao-Mei;Wu, Huan-Gan
以往的研究证实电针对IBS大鼠慢性内脏高敏感性有明显的缓解作用。然而，ST 25是IBS患者的关键穴位，在这些实验中未见报道。8只CVH大鼠在ST 25和ST 37电针20 min，每日1次，连续7 d，模型组和正常组各8只作为对照。第一次电针治疗后，观察腹部退缩反射评分以评估痛阈。采用酶联免疫吸附法（ELISA）测定电针7次后大鼠结肠组织中5-羟色胺（5-HT）、5-HT 3受体（5-HT 3R）和5-HT 4受体（5-HT 4 R）的含量。结果表明：电针可提高CVH大鼠痛阈，降低5-HT含量，升高5-HT_4 R含量，但对5-HT_3R含量无影响。电针治疗CVH大鼠的双配穴选择有待进一步研究。
Previous studies demonstrated the efficacy of electro-acupuncture (EA) in relieving chronic visceral hypersensitivity (CVH) in IBS rats. However, ST25 which is a key acupoint for patients with IBS has not been reported in these experiments. Eight CVH rats were treated by EA at both ST25 and ST37 for 20 min, once daily for seven consecutive days, model rats (n = 8) and normal rats (n = 8) as controls. After the first EA treatment, the abdominal withdrawal reflex scores were investigated to evaluate the pain threshold. After seven EA treatments, the concentrations of 5-hydroxytryptamine (5-HT), 5-HT3 receptor (5-HT3R) and 5-HT4 receptor (5-HT4R) in colon tissue were assayed quantitatively by enzyme-linked immunosorbent assay. The results showed that EA improved the pain threshold of CVH rats, reduced the 5-HT concentration and increased the 5-HT4R concentration, but had no effect on the 5-HT3R concentration. Further studies are needed to optimize the choice of two-matching points for EA in the treatment of CVH rats.