Delayed-type hypersensitivity reaction to paraphenylenediamine is mediated by 2 different pathways of antigen recognition by specific alphabeta human T-cell clones.
Delayed-type hypersensitivity reaction to paraphenylenediamine is mediated by 2 different pathways of antigen recognition by specific alphabeta human T-cell clones.
复制标题
对苯二胺的迟发型超敏反应是由特定 Alphata 人类 T 细胞克隆的 2 种不同的抗原识别途径介导的。
DOI：
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发表时间：
2002
影响因子：
14.2
通讯作者：
B. Blömeke
中科院分区：
文献类型：
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作者：
S. Sieben;Y. Kawakubo;T. Al Masaoudi;H. Merk;B. Blömeke
背景
对苯二胺(PPD)的过敏性接触性皮炎是发病率和职业残疾的常见原因。
客观化
本研究的目的是通过多克隆和单克隆T淋巴细胞培养来表征T细胞对PPD和PPD自氧化产物Bandrowski碱(BB)的反应。
方法
用氚标记的胸腺嘧啶核苷掺入法检测PPD和BB诱导的PBMC和T细胞克隆(TCC)的增殖。用流式细胞仪检测细胞表面标志，用双抗体夹心法检测细胞因子的产生。
结果
除一个例外，TCC为CD4+/CD45RO+，T细胞受体为Alphabeta+。6个TCC中有3个表达Vbeta16。TCC的刺激受HLA-DP限制，TCC分泌IL-4、IL-5和边缘水平的干扰素-γ。TCC对PPD和BB均有反应。BB对TCC的呈递依赖于冲击4h的活的抗原提呈细胞(APC)，固定的APC不能刺激TCC。此外，细胞色素P450酶等代谢活性酶可增强对BB的多克隆反应。BB必须经过代谢和加工。相反，固定APC并不损害它们向TCC呈递PPD的能力，而用PPD脉冲APC则不能刺激TCC。因此，在此过程中必须存在PPD，并且细胞色素不能增强多克隆刺激。
结论
这些结果表明，PPD本身可以被T细胞通过不依赖于加工的途径识别，而它的自氧化产物BB需要加工和可能的代谢来刺激相同的TCC。我们的数据表明，两条不同的抗原提呈途径激活特定的TCC参与了对PPD的免疫反应。
BACKGROUND
Allergic contact dermatitis to paraphenylenediamine (PPD) is a frequent cause of morbidity and occupational disability.
OBJECTIVE
The aim of the study was to characterize T-cell responses to PPD and Bandrowski's base (BB), an autoxidation product of PPD, by using polyclonal and monoclonal T-lymphocyte cultures.
METHODS
PPD- and BB-driven proliferation of PBMCs and T-cell clones (TCCs) was assessed by means of tritiated thymidine incorporation. Surface markers were studied by means of flow cytometry, and cytokine generation was assessed with an ELISA.
RESULTS
TCCs, with one exception, were CD4+/CD45RO+, and T-cell receptors were alphabeta+. Three of 6 TCCs expressed Vbeta 16. TCC stimulation was HLA-DP restricted, and TCCs secreted IL-4, IL-5, and marginal levels of IFN-gamma. TCCs reacted to both PPD and BB. Presentation of BB to TCCs was dependent on viable antigen-presenting cells (APCs) pulsed for 4 hours, and fixed APCs failed to stimulate TCCs. Moreover, polyclonal responses to BB were enhanced by metabolically active enzymes, such as cytochrome P450 enzymes. BB has to be metabolized and processed. In contrast, fixation of APCs did not impair their ability to present PPD to TCC, whereas pulsing of APCs with PPD failed to stimulate TCCs. Thus PPD had to be present during the process, and polyclonal stimulation was not enhanced by cytochromes.
CONCLUSION
These results suggest that PPD itself can be recognized by T cells through a processing-independent pathway, whereas its autoxidation product, BB, required processing and possibly metabolism to stimulate the same TCC. Our data demonstrate that 2 distinct pathways of antigen presentation to activate specific TCCs are involved in the immune response to PPD.
TNP 特异性克隆人 T 细胞系识别的 II 类决定簇。
影响因子：
2.7
作者：
Hanke,JH;Brown,MF;Pollack,MS;Rich,RR
通讯作者：
Rich,RR
通过体内单克隆抗体耗竭揭示 CD4 和 CD8 T 细胞在小鼠接触敏感性中的作用。
DOI：
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发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Gocinski,BL;Tigelaar,RE
通讯作者：
Tigelaar,RE