2.0 MASS-PRODUCTION OF TRICHOGRAMMA MINUTUM RILEY ON FACTITIOUS HOST EGGS
2.0 MASS-PRODUCTION OF TRICHOGRAMMA MINUTUM RILEY ON FACTITIOUS HOST EGGS
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DOI：
10.4039/entm122153010-1
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
J. Laing;G. Eden
中科院分区：
文献类型：
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作者：
J. Laing;G. Eden
开发了一个系统，用于在 Angoumois 谷物蛾 Sitotroga cereella (Olivier) 的卵上生产大量（每周 30 × 10 6 ）的赤眼蜂 Riley。寄生的宿主卵被包装并散装运输，以对抗云杉芽虫、Choristoneura fumiferana (Clemens) 和其他几种鳞翅目害虫。最初尝试以地中海面粉蛾Ephestia kuehniella (Zeller)为寄主，但由于螨类和寄生蜂侵扰、繁殖力低、世代时间长、采蛾过程中需要大量劳动力投入等因素而失败。 S 的蛋。用于饲养宿主的谷物菌在 9°C 下储存长达 1 个月时仍能保持活力，但在 2°C 下储存的卵仅可寄生 15 天。在高湿度（> 70% RH）下饲养宿主，比在低湿度下饲养具有更高的出苗率和侵染率（在一定温度范围内确定了最小短粒虫），并计算了完全发育所需的发育阈值和热量单位积累。使用这些值，在生产的后期阶段，对赤眼蜂进行编程，使其在室温下暴露于光线的 24 小时内出现。该编程有利于寄生蜂在释放之前的运输和处理。
A system was developed to produce large numbers (30 × 10 6 per week) of Trichogramma minutum Riley on eggs of the Angoumois grain moth, Sitotroga cerealella (Olivier). The parasitized host eggs were packaged and shipped in bulk for release against the spruce budworm, Choristoneura fumiferana (Clemens), and several other lepidopterous pests. Initial attempts to use the Mediterranean flour moth, Ephestia kuehniella (Zeller), as a host failed due to such factors as infestation by mites and parasitoids, low fecundity, long generation time, and the necessity for large labour inputs during moth collection. Eggs of S . cerealella , used to rear the host, remained viable when stored for up to 1 month at 9 °C, but eggs stored at 2 °C remained acceptable for parasitism for only 15 days. Rearing the host at high humidity (> 70% RH) gave better percentage emergence and infestation of grain than rearing at low humidity ( T . minutum were determined over a range of temperatures and the developmental threshold and heat unit accumulations necessary for complete development were calculated. Using these values, Trichogramma were programmed, during the latter stages of production, to emerge within 24 h of exposure to light at room temperature. The programming facilitated shipment and handling of the parasitoids prior to their release.