Phenolic fractions from Trifolium pallidum and Trifolium scabrum aerial parts in human plasma protect against changes induced by hyperhomocysteinemia in vitro
Phenolic fractions from Trifolium pallidum and Trifolium scabrum aerial parts in human plasma protect against changes induced by hyperhomocysteinemia in vitro
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DOI：
10.1016/j.fct.2012.07.065
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发表时间：
2012-11-01
影响因子：
4.3
通讯作者：
Olas, Beata
中科院分区：
文献类型：
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作者：
Malinowska, Joanna;Kolodziejczyk-Czepas, Joanna;Olas, Beata
血浆中同型半胱氨酸（homocysteine，Hcy）浓度升高，即高同型半胱氨酸血症，与心血管疾病、神经退行性疾病、肾脏疾病等密切相关。同型半胱氨酸以几种形式存在于人血浆中，包括Hcy的最具反应性的形式-其环状硫酯-同型半胱氨酸硫内酯（HTL），其占血浆总Hcy的高达0.29%。这表明，嘿和HTL也可以作为氧化剂，但各种多酚类抗氧化剂能够抑制同型半胱氨酸或HTL诱导的氧化损伤。我们目前的研究的目的是探讨在体外氧化的变化，在人体血浆中诱导的高同型半胱氨酸血症模型的存在下，从选定的三叶草（三叶草和三叶草地上部分）的酚馏分。高同型半胱氨酸血症刺激减少形式的Hcy（最终剂量100 μ M）或HTL（最终剂量1 μ M）。我们的研究的目的也是解释的酚类馏分对凝血活性的人血浆处理与嘿及其硫代内酯的效果。所测试的酚级分显着抑制氧化应激（测量的总抗氧化剂水平- TAS）在血浆中处理的嘿或HTL。pallidum和T.粗糙还引起由高同型半胱氨酸血症模型诱导的血浆脂质过氧化（通过硫代巴比妥酸反应物质的水平测量）的明显降低。此外，测试的组分调节了用高半胱氨酸及其硫内酯处理的血浆的凝血特性。因此，T. pallidum和T.粗糙的地上部分可能是负责其药用特性在高同型半胱氨酸血症。爱思唯尔有限公司出版
Elevated concentration of homocysteine (Hcy) in human plasma, defined as hyperhomocysteinemia has been correlated with some diseases, such as cardiovascular, neurodegenerative, and kidney disorders. Homocysteine occurs in human plasma in several forms, including the most reactive form of Hcy - its cyclic thioester - homocysteine thiolactone (HTL), which represents up to 0.29% of plasma total Hcy. It is suggested that Hey and HTL may also act as oxidants, but various polyphenolic antioxidants are able to inhibit the oxidative damage induced by Hcy or HTL The aim of our present study was to investigate in vitro oxidative changes in human plasma induced by the model of hyperhomocysteinemia in the presence of the phenolic fractions from selected clovers (Trifolium pallidum and Trifolium scabrum aerial parts). Hyperhomocysteinemia was stimulated by a reduced form of Hcy (final dose 100 mu M) or HTL (final dose 1 mu M). The aim of our study was also to explain the effect of the phenolic fractions on the coagulation activity of human plasma treated with Hey and its thiolactone. Tested phenolic fractions significantly inhibited the oxidative stress (measured by the total antioxidant level - TAS) in plasma treated with Hey or HTL The phenolic fractions from T. pallidum and T. scabrum also caused a distinct reduction of plasma lipid peroxidation (measured by the level of thiobarbituric acid reactive substance) induced by the model of hyperhomocysteinemia. Moreover, tested fractions modulated the coagulation properties of plasma treated with homocysteine and its thiolactone. It seems that antioxidative activities of the phenolic fractions from T. pallidum and T. scabrum aerial parts may be responsible for their medicinal properties during hyperhomocysteinemia. Published by Elsevier Ltd.