Proteomic analysis of responsive stem proteins of resistant and susceptible cashew plants after Lasiodiplodia theobromae infection
Proteomic analysis of responsive stem proteins of resistant and susceptible cashew plants after Lasiodiplodia theobromae infection
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DOI：
10.1016/j.jprot.2014.09.022
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发表时间：
2015-01-15
影响因子：
3.3
通讯作者：
Oliveira, Jose T. A.
中科院分区：
文献类型：
--
作者：
Cipriano, Aline K. A. L.;Gondim, Darcy M. F.;Oliveira, Jose T. A.
流胶病是由坏死营养型真菌可可毛二孢（Lasiodiplodia theobromae（Pat.）Griffon & Maubl威胁到巴西的商业腰果市场。研究腰果对L.在可可树中，在人工感染后的早期对来自商业腰果克隆BRS 226（抗性）的茎进行蛋白质组学分析。此外，还比较了田间条件下生长的抗流胶病腰果植株和自然暴露于病原菌的感流胶病腰果植株的茎蛋白质组谱的变化。双向凝胶电泳（2D-PAGE）后，73个蛋白质显示斑点丰度的统计学显着差异。其中，31个斑点被确定在BRS 226茎与模拟接种的控制和32个茎从田间生长的抗性和敏感腰果植物。L.可可碱应答蛋白主要参与能量代谢途径、胁迫和防御、细胞信号传导和蛋白质代谢，表明真菌感染后对各种细胞功能的调节。由于应激诱导因子似乎对疾病易感性很重要，相对于这些蛋白质丰度的变化可能表明试图维持细胞内稳态，作为抗性决定因子，与氧化爆发的调节作用有关。这些发现提供了第一个信息的细胞机制，在腰果基因型与病理生理状态感染L。生物学意义由一种坏死营养型真菌--可可毛二孢（Lasiodiplodia theobromae）引起的流胶病是巴西东北部半干旱条件下腰果植物的主要病害。尽管对这一致病系统进行了各种研究，但关于腰果与L.可可碱。因此，本原始研究包括腰果茎的差异蛋白质组学分析：（i）抗性矮化克隆BRS 226模拟接种（对照）和人工接种L。可可碱;（ii）腰果植株对流胶病具有抗性和敏感性，来源于BRS 226在高发病率的商业果园中的自由授粉。重组蛋白在腰果受L.可可碱在鉴定与响应L.可可碱感染此外，这项研究可能有助于改善育种计划，旨在选择抗/耐受腰果无性系对这种病原体。(C)2014爱思唯尔有限公司版权所有。
Gummosis is an aggressive disease caused by the necrotrophic fungus Lasiodiplodia theobromae (Pat.) Griffon & Maubl that threatens commercial cashew orchads in Brazil. To study the molecular mechanisms involved in the cashew response to L. theobromae, a proteomic analysis of stems from the commercial cashew clone BRS 226 (resistant) was conducted at early times post-artificial infection. In addition, changes in the stem proteome profiles of gummosis resistant and susceptible cashew plants grown under field condition and naturally exposed to pathogen were also compared. After two-dimensional gel electrophoresis (2D-PAGE), 73 proteins showed statistically significant differences in spot abundance. Of these, 31 spots were identified in BRS 226 stems compared with mock-inoculated controls and 32 in stems collected from field-grown resistant and susceptible cashew plants. L. theobrornae-responsive proteins were mainly involved in energy metabolism pathways, stress and defense, cell signaling and protein metabolism indicating modulation of various cellular functions upon fungal infection. As stress-inducing factors seem to be important for susceptibility to disease, the change in the abundance relative these proteins may possibly indicate an attempt to maintain cellular homeostasis, as resistance determinant factor, related with a possible role in the regulation of oxidative burst. These findings provide the first information about the cellular mechanisms acting in the Anacardium occidentale genotypes associated with the pathophysiological state of infection with L. theobromae.Biological significanceGummosis caused by Lasiodiplodia theobromae, a necrotrophic fungus, is the major disease of cashew plants in the semi-arid conditions of northeastern Brazil. Although various studies were carried out on this pathosystem, there is no information available on the molecular mechanisms of plant defense related to the incompatible interaction of cashew with L. theobromae. Therefore, this original study comprises a differential proteomic analysis of cashew sterns from: (i) resistant dwarf clone BRS 226 mock-inoculated (control) and artificially inoculated with L. theobromae; and (ii) cashew plants bearing resistant and susceptible traits to gummosis, originated from open pollination of BRS 226 in a commercial orchard with high disease incidence. The contribution of the reprogrammed proteins to molecular events triggered in cashew plants challenged by L. theobromae has a great relevance in the identification of the host candidate proteins linked to biological pathways that respond to L. theobromae infection. Furthermore this study may contribute to improve breeding programs aimed at selecting resistant/tolerant cashew clones toward this pathogen. (C) 2014 Elsevier B.V. All rights reserved.