Testosterone inhibits gonadotropin-releasing hormone pulse frequency in the male sheep.
Testosterone inhibits gonadotropin-releasing hormone pulse frequency in the male sheep.
复制标题
睾酮抑制雄性绵羊促性腺激素释放激素的脉冲频率。
DOI：
10.1095/biolreprod45.1.188
复制
发表时间：
1991
影响因子：
3.6
通讯作者：
Rhim,TJ
中科院分区：
文献类型：
--
作者：
Jackson,GL;Kuehl,D;Rhim,TJ
目的是确定慢性睾酮(T)治疗对GnRH和LH分泌的影响。公羊要么被单独阉割，要么被阉割后立即用含有t的硅胶植入物治疗。几周后，通过手术植入一个收集垂体门静脉血液的装置。6 ~ 7天后，对有意识动物的门脉和颈静脉同时连续采血。样品每隔10分钟分一次，持续6 ~ 12小时。收集结束前1小时，所有动物静脉注射250 ng GnRH。在同时采集的6对动物样本中，T降低了GnRH脉冲频率（1.8±0.2比0.9±0.3/h,p< 0.03）和LH脉冲频率（1.6±0.1比0.8±0.3/h,p< 0.03）。T不改变GnRH或LH脉冲的振幅。睾酮降低GnRH平均值（9.7±0.6 vs. 7.9±0.5 pg/ml,p< 0.05），而LH平均值无显著降低（9.6±1.4 vs. 6.1±1.8 ng/ml,p= 0.16）。这些结果支持了T降低GnRH脉冲频率的假设。
The objective was to determine the effect of chronic testosterone (T) treatment on GnRH and LH secretion in wethers. Rams were either castrated only or castrated and immediately treated with Silastic implants containing T. Several weeks later, a device for collecting hypophyseal-portal blood was surgically implanted. Six to seven days later, blood samples were collected simultaneously and continuously from the portal vessels and jugular vein of pairs of conscious animals. Samples were divided at 10-min intervals for 6−12 h. One hour before the end of collection, all animals received i.v. injections of 250 ng of GnRH. In samples collected simultaneously from 6 pairs of animals, T reduced the frequency of both GnRH pulses (1.8 ± 0.2 vs. 0.9 ± 0.3/h,p< 0.03) and LH pulses (1.6 ± 0.1 vs. 0.8 ± 0.3/h,p< 0.03). T did not alter amplitude of either GnRH or LH pulses. Testosterone reduced mean GnRH (9.7 ± 0.6 vs. 7.9 ± 0.5 pg/ml,p< 0.05), whereas mean LH was not significantly reduced (9.6 ± 1.4 vs. 6.1 ± 1.8 ng/ml,p= 0.16). These results support the hypothesis that T reduces GnRH pulse frequency.