Dependence of the encapsidation function of the adenovirus L1 52/55-kilodalton protein on its ability to bind the packaging sequence.
Dependence of the encapsidation function of the adenovirus L1 52/55-kilodalton protein on its ability to bind the packaging sequence.
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腺病毒 L1 52/55-千道尔顿蛋白的衣壳化功能对其结合包装序列的能力的依赖性。
DOI：
10.1128/jvi.80.4.1965-1971.2006
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Imperiale,MichaelJ
中科院分区：
文献类型：
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作者：
Perez-Romero,Pilar;Gustin,KurtE;Imperiale,MichaelJ
腺病毒IVa 2和L1 52/55-kDa蛋白参与新病毒颗粒的组装。这两种蛋白质都与病毒染色体的包装序列结合，并且任一蛋白质的表达缺乏导致没有病毒后代：L1 52/55-kDa蛋白质的缺乏导致仅形成空衣壳，IVa 2蛋白质的缺乏导致没有衣壳组装。此外，IVa 2和L1 52/55-kDa蛋白在腺病毒感染期间相互作用。然而，目前尚不清楚的是，这种相互作用在病毒感染过程中何时以及如何发生。我们通过构建L1 52/55-kDa蛋白截短体，确定了L1 52/55-kDa蛋白与IVa 2蛋白相互作用、DNA结合和病毒复制所需的结构域。我们发现L1 52/55-kDa蛋白的N-末端173个氨基酸对于与IVa 2蛋白的相互作用是必不可少的。然而，对于不表达L1 52/55-kDa蛋白的pm 8001突变病毒的DNA结合和互补，L1 52/55-kDa蛋白的氨基末端331个氨基酸是必需的。这些结果表明，感染性病毒颗粒的产生取决于L1 52/55-kDa蛋白结合DNA的能力。
The adenovirus IVa2 and L1 52/55-kDa proteins are involved in the assembly of new virus particles. Both proteins bind to the packaging sequence of the viral chromosome, and the lack of expression of either protein results in no virus progeny: the absence of the L1 52/55-kDa protein leads to formation of only empty capsids, and the absence of the IVa2 protein results in no capsid assembly. Furthermore, the IVa2 and L1 52/55-kDa proteins interact with each other during adenovirus infection. However, what is not yet clear is when and how this interaction occurs during the course of the viral infection. We defined the domains of the L1 52/55-kDa protein required for interaction with the IVa2 protein, DNA binding, and virus replication by constructing L1 52/55-kDa protein truncations. We found that the N-terminal 173 amino acids of the L1 52/55-kDa protein are essential for interaction with the IVa2 protein. However, for both DNA binding and complementation of thepm8001 mutant virus, which does not express the L1 52/55-kDa protein, the amino-terminal 331 amino acids of the L1 52/55-kDa protein are necessary. These results suggest that the production of infectious virus particles depends on the ability of the L1 52/55-kDa protein to bind to DNA.