Environmentally friendly surface modification of PDMS using PEG polymer brush
Environmentally friendly surface modification of PDMS using PEG polymer brush
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DOI：
10.1002/elps.200900132
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发表时间：
2009-09
期刊：
影响因子：
2.9
通讯作者：
Zhaowei Zhang;Xiaojun Feng;Qingming Luo;Bifeng Liu
中科院分区：
文献类型：
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作者：
Zhaowei Zhang;Xiaojun Feng;Qingming Luo;Bifeng Liu
研究了一种基于PEG - NH2的环境友好型PDMS微芯片表面改性策略，以防止蛋白质吸附并提高分离性能。采用改进的合成方法合成了PEG‐NH2。采用两步接枝法对PDMS进行改性。通过衰减全反射的FTIR吸收和接触角测量验证了PEG - NH2成功接枝到PDMS表面。随后的EOF测量和PEG修饰的PDMS微芯片的蛋白质吸附研究表明，在超过30天的时间里，EOF对非特异性蛋白质吸附有明显的抑制和抗性。进一步证明了四种FITC标记氨基酸的分离具有高重复性和再现性。比较3 -（2 -呋喃）喹啉- 2 -甲醛标记的牛血清蛋白在PDMS微芯片表面修饰前后的电泳结果表明，PEG修饰的PDMS微芯片的电泳性能显著提高，表明我们的PEG接枝方法成功地修饰了PDMS的表面特性，并防止了蛋白质的吸附。我们期望这种环境友好的表面修饰方法将有助于未来具有长期表面稳定性的蛋白质分离。
A PEG‐NH2‐based environmentally friendly surface modification strategy was developed for PDMS microchips to prevent protein adsorption and to enhance separation performance. PEG‐NH2 was synthesized using a modified synthesis procedure. A two‐step grafting method was used for PDMS modification. FTIR absorption by attenuated total reflection and contact angle measurements verified the successful grafting of PEG‐NH2 onto the PDMS surface. Subsequent EOF Measurements and protein adsorption studies of PEG‐modified PDMS microchips revealed noticeable EOF suppression and resistance to nonspecific protein adsorption for more than 30 days. Separation of four FITC‐labeled amino acids was further demonstrated with high repeatability and reproducibility. Comparison of electrophoresis of 3‐(2‐furoyl)quinoline‐2‐carboxaldehyde‐labeled BSA using PDMS microchips before and after surface modification resulted in significantly improved electrophoretic performance of the PEG‐modified PDMS microchips, suggesting that our PEG grafting method successfully modified PDMS surface property and prevented adsorption of proteins. We expect that this environmentally friendly surface modification method will be useful for future protein separations with long‐term surface stability.