Effects of furfural and acetic acid on growth and lipid production from glucose and xylose by Rhodotorula glutinis
Effects of furfural and acetic acid on growth and lipid production from glucose and xylose by Rhodotorula glutinis
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DOI：
10.1016/j.biombioe.2010.10.009
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发表时间：
2011-01-01
影响因子：
6
通讯作者：
Paraschivescu, Maria
中科院分区：
文献类型：
--
作者：
Zhang, Guochang;French, William Todd;Paraschivescu, Maria
使用产油酵母红酵母（ATCC 15125）研究了木质纤维素糖向三酰基甘油（生物柴油或可再生柴油原料）的微生物转化。在摇瓶实验中，R.首先在富含氮的培养基中利用木质纤维素生物质柳枝稷的人工酸水解产物作为唯一的碳源和能量源生长拟南芥。一旦培养物达到稳定期，收获细胞并转移到含有人工酸水解产物糖的新鲜无氮培养基中用于脂质积累。对收集的数据进行分析表明，酵母能够在含有人工酸水解物糖作为碳源和能源的培养基中生长。净比生长速率表明，人工酸水解液中乙酸和糠醛的存在抑制了R. glutinis对葡萄糖的生长有抑制作用，但对木糖的生长无抑制作用。用重量法测定细胞内脂质的积累量，从最初的4.3%增加到39.0%。积累的脂肪酸主要为棕榈酸、硬脂酸、油酸、亚油酸和γ-亚油酸。这些结果表明，利用R.是的。(c)2010爱思唯尔有限公司版权所有。
Microbial conversion of lignocellulosic sugars to triacylglycerols (a biodiesel or renewable diesel feedstock) was investigated using the oleaginous yeast Rhodotorula glutinis (ATCC 15125). In the shake flask experiments, R. glutinis was first grown in a nitrogen-rich medium utilizing an artificial acid hydrolysate of lignocellulosic biomass switchgrass as the sole carbon and energy source. Once the culture had reached the stationary phase, the cells were harvested and transferred to a fresh nitrogen-free media containing artificial acid hydrolysate sugars for lipid accumulation. Analysis of the data collected showed that the yeast were able to grow in the medium containing artificial acid hydrolysate sugars as the carbon and energy source. The net specific Growth rate(s) indicated that the presence of acetic acid and furfural in the artificial acid hydrolysate inhibited the growth of R. glutinis on glucose, but not the growth on xylose. The lipid accumulated in the cells, determined by gravimetrical method, increased from initial 4.3%-39.0% of dry cell mass weight. The major fatty acids of the accumulated lipids were palmitic acid, stearic acid, oleic acid, linoleic acid and gamma-linoleic acid. These results indicate that it is feasible to convert the sugars in acid hydrolysate of lignocellulosic biomass to triacylglycerols using R. glutinis. (c) 2010 Elsevier Ltd. All rights reserved.