Prevalence of a non-male-killing spiroplasma in natural populations of Drosophila hydei
Prevalence of a non-male-killing spiroplasma in natural populations of Drosophila hydei
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DOI：
10.1128/aem.00803-06
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发表时间：
2006-10-01
影响因子：
4.4
通讯作者：
Fukatsu, Takema
中科院分区：
文献类型：
--
作者：
Kageyama, Daisuke;Anbutsu, Hisashi;Fukatsu, Takema
在多种昆虫中发现雄性杀死表型，并且通常与母系遗传的内共生细菌有关。在一些种类的果蝇中，发现了低频率（0.1 - 3%）的螺原体属内共生体杀死雄性。在这项研究中，没有引起雄性死亡的螺旋体感染在日本的果蝇种群中很普遍（23%至66%）。分子系统发育分析表明，该菌株感染了一株螺原体，该菌株与其他果蝇种的杀雄螺原体密切相关。人工转移实验表明，螺原体基因型而不是宿主基因型是导致雄性杀伤表型缺失的原因。螺旋体在自然宿主d.h dei和人工宿主Drosophila melanogaster中的感染密度均显著低于杀雄螺旋体NSRO，这与螺旋体诱导的杀雄表达需要一个阈值感染密度的假设相一致。
Male-killing phenotypes are found in a variety of insects and are often associated with maternally inherited endosymbiotic bacteria. In several species of Drosophila, male-killing endosymbionts of the genus Spiroplasma have been found at low frequencies (0.1 to 3%). In this study, spiroplasma infection without causing male-killing was shown to be prevalent (23 to 66%) in Japanese populations of Drosophila hydei. Molecular phylogenetic analyses showed that D. hydei was infected with a single strain of spiroplasma, which was closely related to male-killing spiroplasmas from other Drosophila species. Artificial-transfer experiments suggested that the spiroplasma genotype rather than the host genotype was responsible for the absence of the male-killing phenotype. Infection densities of the spiroplasma in the natural host, D. hydei, and in the artificial host, Drosophila melanogaster, were significantly lower than those of the male-killing spiroplasma NSRO, which was in accordance with the hypothesis that a threshold infection density is needed for the spiroplasma-induced male-killing expression.