Dietary Platycodon grandiflorus Attenuates Hepatic Insulin Resistance and Oxidative Stress in High-Fat-Diet Induced Non-Alcoholic Fatty Liver Disease
Dietary Platycodon grandiflorus Attenuates Hepatic Insulin Resistance and Oxidative Stress in High-Fat-Diet Induced Non-Alcoholic Fatty Liver Disease
复制标题
桔梗对高脂饮食诱导的非酒精性脂肪性肝病大鼠肝脏胰岛素抵抗和氧化应激的影响
DOI：
10.3390/nu12020480
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发表时间：
2020-02-01
期刊：
影响因子：
5.9
通讯作者：
Chen, Fang
中科院分区：
文献类型：
--
作者：
Ke, Weixin;Wang, Pan;Chen, Fang
桔梗的根具有保肝和抗氧化的作用，在亚洲有着悠久的食用和药用历史。然而，PG抗非酒精性脂肪性肝病（NAFLD）的机制尚不清楚。本研究的目的是探讨PG抑制高脂饮食（HFD）诱导的小鼠NAFLD的机制。雄性C57 BL/6 J小鼠用标准食物或补充有或没有PG的HFD喂养16周。测定血脂、肝脏脂肪变性、氧化应激和胰岛素敏感性。分析相关通路中肝酶的表达或活性，探讨其作用机制。PG通过调节乙酰辅酶A羧化酶（p-ACC）的磷酸化和脂肪酸合成酶（FAS）的表达，显著减轻HFD诱导的肝损伤和高脂血症，以及肝脂肪变性。此外，PG通过恢复谷胱甘肽（GSH）含量和抗氧化活性改善氧化应激，并通过调节PI 3 K/Akt/GSK 3 β信号通路改善胰岛素敏感性。我们的数据表明，饮食PG对肝脏胰岛素敏感性和氧化应激有深远的影响，这是NAFLD发病机制中的两个关键因素，表明PG作为NAFLD治疗策略的潜力。
The root of Platycodon grandiflorus (PG), with hepatoprotective and anti-oxidation effects, has a long history of being used as food and herbal medicine in Asia. However, the mechanism of PG against non-alcoholic fatty liver disease (NAFLD) is still not clear. The aim of this study was to investigate the mechanism of PG suppressing the development of NAFLD induced by a high-fat diet (HFD) in mice. Male C57BL/6J mice were fed with either a standard chow diet or a HFD, either supplemented with or without PG, for 16 weeks. Serum lipids, liver steatosis, oxidative stress and insulin sensitivity were determined. Expressions or activities of hepatic enzymes in the related pathways were analyzed to investigate the mechanisms. PG significantly reduced HFD-induced hepatic injury and hyperlipidemia, as well as hepatic steatosis via regulating phosphorylation of acetyl-CoA carboxylase (p-ACC) and expression of fatty acid synthase (FAS). In addition, PG ameliorated oxidative stress by restoring glutathione (GSH) content and antioxidant activities, and improved insulin sensitivity by regulating the PI3K/Akt/GSK3 beta signaling pathway. Our data showed that dietary PG have profound effects on hepatic insulin sensitivity and oxidative stress, two key factors in the pathogenesis of NAFLD, demonstrating the potential of PG as a therapeutic strategy for NAFLD.