Rapid simultaneous detection of fumonisin B1 and deoxynivalenol in grain by immunochromatographic test strip
Rapid simultaneous detection of fumonisin B1 and deoxynivalenol in grain by immunochromatographic test strip
复制标题
免疫层析试纸条快速同时检测谷物中伏马菌素B1和脱氧雪腐镰刀菌烯醇
DOI：
10.1016/j.ab.2020.113878
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发表时间：
2020-10-01
影响因子：
2.9
通讯作者：
Huang, Zhibing
中科院分区：
文献类型：
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作者：
Huang, Xi;Huang, Xinyu;Huang, Zhibing
真菌毒素，如伏马菌素B-1 （FB1）和脱氧雪腐镰刀菌醇（DON），是真菌产生的有毒次生代谢物。本研究采用免疫层析法同时检测粮食样品中FB1和DON（均为水溶性），以检测这两种真菌毒素的含量是否超过1 mg/kg的限量。为此开发了两种类型的免疫层析试纸条（ics），即以金纳米球（通过柠檬酸三钠还原制备）和金纳米花（通过金种子介导生长制备）为标记物，其对FB1和DON的敏感性分别为20和5.0 ng/mL。前者用于51份小麦样品和18份玉米样品中FB1和DON的检测，结果与高效液相色谱法和酶联免疫吸附法一致。该技术适用于大样本FB1和DON的现场筛选。
Mycotoxins, such as fumonisin B-1 (FB1) and deoxynivalenol (DON), are toxic secondary metabolites produced by fungi. Herein, the simultaneous detection of FB1 and DON (both of which are water-soluble) in grain samples by immunochromatographic methods was used to probe whether the levels of these mycotoxins exceeded the limit of 1 mg/kg. Two types of immunochromatographic test strips (ICSs) were developed for this purpose, namely, those based on Au nanospheres (prepared via reduction with trisodium citrate) and Au nanoflowers (prepared by Au seed-mediated growth) as markers, with the respective sensitivities to both FB1 and DON equalling 20 and 5.0 ng/mL. The former ICS was used to detect FB1 and DON in grain (51 wheat samples and 18 maize samples), providing results consistent with those of high-performance liquid chromatography and enzyme-linked immunosorbent assays. The developed technique was suitable for the on-site screening of large-scale samples for FB1 and DON.