Oxidative injury induced by the herbicide quinclorac on Echinochloa oryzicola Vasing. and the involvement of antioxidative ability in its highly selective action in grass species
Oxidative injury induced by the herbicide quinclorac on Echinochloa oryzicola Vasing. and the involvement of antioxidative ability in its highly selective action in grass species
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DOI：
10.1016/j.plantsci.2004.05.005
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发表时间：
2004-09
期刊：
影响因子：
5.2
通讯作者：
Y. Sunohara;H. Matsumoto
中科院分区：
文献类型：
--
作者：
Y. Sunohara;H. Matsumoto
研究抗氧化能力可能参与除草剂二氯喹啉酸（3,7-二氯-8-喹啉甲酸）对草类的高度选择性作用、二氯喹啉酸诱导的氧化损伤以及抗氧化酶超氧化物歧化酶（SOD，EC 1.15.1.1）、过氧化氢酶（CAT，EC 1.11.1.6）、抗坏血酸过氧化物酶（APX，EC）的活性1.11.1.11) 和谷胱甘肽还原酶 (GR, EC 1.6.4.2) 在耐性水稻 (Oryza sativa L. cv. Nipponbare) 和特别敏感的禾本科杂草稗 (Echinochloa oryzicola Vasing) 中进行了研究。在连续光照和黑暗条件下，1μM 二氯喹啉酸处理的水稻幼苗和对照水稻幼苗的总叶绿素含量、硫代巴比妥酸反应物质（TBARS）的形成以及乙烷和乙烯的产生没有显着差异。相反，在连续光照 24 小时的二氯喹啉酸处理的稻米幼苗中，叶片中的叶绿素含量下降，芽中 TBARS 的形成和乙烷的释放增加。这些变化在黑暗中没有观察到，表明光增强了二氯喹啉酸在水稻和稻米中的选择性活性。 α-生育酚 (α-TOH) 是一种自由基清除剂，可显着抑制二氯喹啉酸诱导的 E. oryzicola 芽中 TBARS 的形成和叶绿素损失。抗氧化酶测定表明，稻米地上部SOD、CAT、APX、GR酶的组成活性均低于水稻。此外，水稻、玉米（Zea mays L. cv. Honey Bantam）、E. oryzicola、E. crus-galli Beauv之后，地上部APX和GR的组成活性与叶片中叶绿素含量（对照的%）之间存在显着相关性。变种crus-galli 和 E. crus-galli var.用二氯喹啉酸处理福尔摩沙奥威。这些结果表明，遗传抗氧化能力的差异是导致水稻和稻瘟病菌对二氯喹啉酸具有特别高选择性的因素之一。
To investigate the possible involvement of antioxidative ability in the highly selective action of the herbicide quinclorac (3,7-dichloro-8-quinolinecarboxylic acid) in grass species, quinclorac-induced oxidative injuries and the activities of the antioxidative enzymes superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), ascorbic acid peroxidase (APX, EC 1.11.1.11), and glutathione reductase (GR, EC 1.6.4.2) were studied in tolerant rice (Oryza sativa L. cv. Nipponbare) and a particularly susceptible grass weed, Echinochloa oryzicola Vasing. There were no significant differences in total chlorophyll content, formation of thiobarbituric acid-reactive substances (TBARS), and ethane and ethylene productions between 1μM quinclorac-treated and control seedlings of rice both under continuous light and dark conditions. In contrast, the chlorophyll content in leaves decreased, and TBARS formation in shoots and ethane evolution increased in quinclorac-treated E. oryzicola seedlings under continuous light for 24h. These changes were not observed in the dark, indicating that light enhanced the selective activity of quinclorac between rice and E. oryzicola. α-Tocopherol (α-TOH), a free radical scavenger, significantly suppressed quinclorac-induced TBARS formation in shoots and chlorophyll loss in the leaves of E. oryzicola. Antioxidant enzyme assays showed that the constitutive activities of SOD, CAT, APX, and GR enzymes in the shoots of E. oryzicola were lower than those in rice. Furthermore, there were significant correlations between the constitutive activities of APX and GR in shoots and chlorophyll content (% of control) in leaves after rice, corn (Zea mays L. cv. Honey Bantam), E. oryzicola, E. crus-galli Beauv. var. crus-galli, and E. crus-galli var. formosensis Ohwi were treated with quinclorac. These results suggest that the difference in hereditary antioxidative ability is one factor that results in the particularly high selectivity of quinclorac between rice and E. oryzicola.