Cough-generated aerosols of Mycobacterium tuberculosis -: A new method to study infectiousness
Cough-generated aerosols of Mycobacterium tuberculosis -: A new method to study infectiousness
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DOI：
10.1164/rccm.200308-1101oc
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发表时间：
2004-03-01
影响因子：
24.7
通讯作者：
Heifets, LB
中科院分区：
文献类型：
--
作者：
Fennelly, KP;Martyny, JW;Heifets, LB
结核病患者产生的传染性气溶胶的浓度和大小分布从未被直接测量过。我们旨在评估我们开发的收集和量化可培养的结核分枝杆菌咳嗽产生的气雾剂的方法的可行性。研究对象从一家转诊医院招募，大多数患有耐多药结核病。他们对着一个装有微生物空气采样器的房间咳嗽，同时在两次5分钟的会议中测量咳嗽频率。咳嗽产生的气溶胶培养在16名涂片阳性肺结核患者中有4名（25%）呈阳性。在有效治疗的前三周内，咳嗽产生的气溶胶培养物迅速减少。咳嗽气雾剂培养阳性与前一周缺乏治疗相关（p = 0.007），与咳嗽频率相关（p = 0.08）。这些气溶胶的粒径分布是可变的，但大多数粒径在可吸入范围内。量化咳嗽产生的气溶胶是可行的，并为研究结核分枝杆菌和其他空气传播病原体的传染性和传播提供了新的方法。
The concentration and size distribution of infectious aerosols produced by patients with pulmonary tuberculosis (TB) has never been directly measured. We aimed to assess the feasibility of a method that we developed to collect and quantify culturable cough-generated aerosols of Mycobacterium tuberculosis. Subjects were recruited from a referral hospital and most had multidrug-resistant TB. They coughed into a chamber containing microbial air samplers while cough frequency was measured during two 5-minute sessions. Cough-generated aerosol cultures were positive in 4 of 16 subjects (25%) with smear-positive pulmonary TB. There was a rapid decrease in the cough-generated aerosol cultures within the first 3 weeks of effective treatment. Culture-positive cough aerosols were associated with lack of treatment during the previous week (p = 0.007), and there was a trend in the association with cough frequency (p = 0.08). The size distributions of these aerosols were variable, but most particle sizes were in the respirable range. Quantification of viable cough-generated aerosols is feasible and offers a new approach to study infectiousness and transmission of M. tuberculosis and other airborne pathogens.