Study of in vivo differentiation of rat bone marrow stromal cells into Schwann cell-like cells
Study of in vivo differentiation of rat bone marrow stromal cells into Schwann cell-like cells
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DOI：
10.1002/micr.20184
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发表时间：
2006-01-01
期刊：
影响因子：
2.1
通讯作者：
Gu, XS
中科院分区：
文献类型：
--
作者：
Chen, X;Wang, XD;Gu, XS
为了建立一个丰富的雪旺细胞（Schwann cells，SC）库，作为构建组织工程化神经移植物的种子细胞，我们研究了骨髓基质细胞（bone marrow stromal cells，MSCs）体内诱导分化为SC样细胞的可行性。在这项研究中，从成年大鼠的骨髓中收获MSC，培养扩增，并进行表征。将传代培养的MSCs用Hoechst 33342标记后，移植到由硅胶管桥接大鼠坐骨神经缺损制成的神经再生室中。术后4周，部分分化的MSC转化为S-100蛋白免疫阳性的SC样细胞，并伴有再生神经纤维的髓鞘形成。步行跟踪分析提供的证据表明，骨髓间充质干细胞移植有助于坐骨神经的重建和靶组织的神经再支配。实验结果表明，骨髓间充质干细胞能够在体内分化为干细胞样细胞，使其成为一个有前途的候选细胞移植在周围神经修复。(c)2006 Wiley-Liss，Inc.
In order to raise an abundant and accessible reservoir for Schwann cells (SCs), which are used as seed cells for constructing tissue-engineered nerve grafts, we investigated the feasibilty of in vivo differentiation of bone marrow stromal cells (MSCs) into SC-like cells. In this study, MSCs were harvested from adult rats' bone marrow, culture-expanded, and characterized. Subcultured MSCs were then labeled with Hoechst 33342, followed by transplantation into the nerve regeneration chamber, which was made of a silicone tube bridging the sciatic nerve defect of the rats. Four weeks after surgery, some of the differentiated MSCs turned into SC-like cells immunopositive to S-100 protein, accompanied by myelination of the regenerated nerve fibers. Walking-track analyses provided evidence that transplantation of MSCs contributed to reconstruction of the sciatic nerve and reinnervation of target tissues. The experimental results suggest that MSCs are capable of differentiating into SC-like cells in vivo, making them a promising candidate for cell transplantation in peripheral nerve repair. (c) 2006 Wiley-Liss, Inc.