Characterization of a novel human UDP-GalNAc transferase, pp-GalNAc-T10
Characterization of a novel human UDP-GalNAc transferase, pp-GalNAc-T10
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DOI：
10.1016/s0014-5793(02)03399-9
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发表时间：
2002-11-06
期刊：
影响因子：
3.5
通讯作者：
Narimatsu, H
中科院分区：
文献类型：
--
作者：
Cheng, LM;Tachibana, K;Narimatsu, H
克隆了一个新的人多肽n -乙酰半乳糖胺转移酶（pp-GalNAc-T）基因家族成员，与人pp-GalNAc-T7同源，命名为pp-GalNAe-T10。在小肠、胃、胰腺、卵巢、甲状腺和脾脏中均发现了pp-GalNAc-T10转录本。在多肽GalNAc转移实验中，重组pp-GalNAc-T10将GalNAc转移到粘蛋白衍生的肽底物上。此外，pp-GalNAe-T10与糖肽底物表现出很强的转移酶活性。(C) 2002年欧洲生化学会联合会。Elsevier Science B.V.版权所有。
A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) gene family was cloned as a homolog of human pp-GalNAc-T7, and designated pp-GalNAe-T10. pp-GalNAc-T10 transcript was found in the small intestine, stomach, pancreas, ovary, thyroid gland and spleen. In a polypeptide GalNAc-transfer assay, recombinant pp-GalNAc-T10 transferred GalNAc onto a panel of mucin-derived peptide substrates. Furthermore, pp-GalNAe-T10 demonstrated strong transferase activity with glycopeptide substrates. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.