Cadherin-10 is a novel blood-brain barrier adhesion molecule in human and mouse
Cadherin-10 is a novel blood-brain barrier adhesion molecule in human and mouse
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DOI：
10.1016/j.brainres.2005.07.078
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发表时间：
2005-10-05
期刊：
影响因子：
2.9
通讯作者：
Clark, P
中科院分区：
文献类型：
--
作者：
Williams, MJ;Lowrie, MB;Clark, P
维持中枢神经系统的特殊环境需要脑微血管内皮细胞(血脑屏障)或脑室衬里上皮(脑脊液屏障)或脉络丛(血脑脊液屏障)提供屏障。血管内皮细胞间连接在血脑屏障中比在其他组织中更为广泛，具有精细的紧密连接。然而，这些连接的分子组成几乎没有什么不同。在这里，我们发现，在人脑和小鼠脑中，II型经典钙粘蛋白-10在血脑屏障和视网膜内皮细胞中表达，但在脑室周围器官(CVO)的渗漏微血管或非中枢组织中不表达。这种表达模式与VE-钙粘蛋白不同，也与VE-钙粘蛋白相反，VE-钙粘蛋白在紧密的皮质微血管中减少或缺失，但在漏水的CVO血管中存在。在CVO中，屏障功能从微血管系统切换到邻近的心室上皮细胞，我们也发现这些上皮细胞表达钙粘素-10。在血脑屏障缺失的多形性胶质母细胞瘤的血管中，未检测到钙粘附素-10。这种钙粘附素-10独特的表达模式表明，它在脑屏障的发展和维持中起着关键作用。(C)2005 Elsevier B.V.保留所有权利。
Maintenance of the specialised environment of the central nervous system requires barriers provided by the endothelium of brain microvessels (the blood-brain barrier (BBB)) or the epithelium lining the ventricles (CSF-brain barrier) or the choroid plexus (blood-CSF barrier). Inter-endothelial junctions are more extensive in the BBB than in other tissues, with elaborate tight junctions. However, few differences in the molecular composition of these junctions have been described. Here, we show, in both human and mouse brain, that the type II classical cadherin, cadherin-10, is expressed in BBB and retinal endothelia, but not in the leaky microvessels of brain circumventricular organs (CVO), or in those of non-CNS tissues. This expression pattern is distinct from, and reciprocal to, VE-cadherin, which is reduced or absent in tight cortical microvessels, but present in leaky CVO vessels. In CVO, the barrier function is switched from the microvasculature to the adjacent ventricular epithelium, which we also find to express cadherin-10. In the vessels of gliobastoma multiforme tumours, where BBB is lost, cadherin-10 is not detected. This demonstration of a distinctive expression pattern of cadherin-10 suggests that it has a pivotal role in the development and maintenance of brain barriers. (c) 2005 Elsevier B.V. All rights reserved.