Tanshinone IIA, an ingredient of Salvia miltiorrhiza BUNGE, induces apoptosis in human leukemia cell lines through the activation of caspase-3
Tanshinone IIA, an ingredient of Salvia miltiorrhiza BUNGE, induces apoptosis in human leukemia cell lines through the activation of caspase-3
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DOI：
10.1038/emm.1999.28
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发表时间：
1999-12-31
影响因子：
12.8
通讯作者：
An, KS
中科院分区：
文献类型：
--
作者：
Sung, HJ;Choi, SM;An, KS
丹参酮II-A是从丹参中分离的菲醌衍生物，丹参是一种传统的草药，已知具有诱导衰老、抗氧化和细胞毒性活性。我们研究了丹参酮Ⅱ-A对HL 60人早幼粒细胞白血病细胞和K562人红白血病细胞的细胞效应。丹参酮II-A诱导的剂量和时间依赖性DNA片段成180 bp的倍数和特定的蛋白水解裂解的聚（ADP-核糖）聚合酶在两个细胞系。丹参酮Ⅱ-A处理后的K562细胞的PI染色和流式细胞术分析显示具有亚二倍体DNA的细胞增加，指示细胞的凋亡状态。丹参酮II-A处理HL 60和K562细胞后，Caspase-3活性显著升高，而Caspase-1活性无明显变化。提示丹参酮Ⅱ A诱导HL 60和K562细胞凋亡可能与caspase家族成员的选择性有关。
Tanshinone II-A is a derivative of phenanthrenequinone isolated from Salvia miltiorrhiza BUNGE, a traditional herbal medicine that is known to induce antiinflammatory, anti-oxidative and cytotoxic activity. We have examined cellular effects of Tanshione II-A on HL60 human promyelocytic leukemic cells and K562 human erythroleukemic cells. Tanshione II-A induced a dose- and time-dependent DNA fragmentation into the multiples of 180 bp and specific proteolytic cleavage of poly(ADP-ribose) polymerase in both cell lines. PI-staining and flow cytometry analysis of K562 cells following Tanshione II-A treatment showed an increase of the cells possessing hypodiploid DNA indicative of apoptotic state of cells. Caspase-3 activity was significantly increased during Tanshinone II-A treatment of both HL60 and K562 cells, whereas caspase-1 activity was not changed. These results suggest that Tanshione II-A induced HL60 and K562 cellular apoptosis that may be associated with the selective members of caspase family.