Cancer cells ablation with irreversible electroporation
Cancer cells ablation with irreversible electroporation
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DOI：
10.1177/153303460500400615
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发表时间：
2005-12-01
影响因子：
2.8
通讯作者：
Rubinsky, B
中科院分区：
文献类型：
--
作者：
Miller, L;Leor, J;Rubinsky, B
在这项研究中，我们对人肝癌细胞(HepG2)进行了体外不可逆电穿孔(IRE)实验，以探讨IRE作为一种治疗不良组织的新技术。不可逆电穿孔(IRE)是通过施加微秒到毫秒的电脉冲使细胞膜不可逆地通透。到目前为止，在基因治疗和电化疗中使用可逆电穿孔时，IRE只被作为一种不受欢迎的条件进行研究。存在一种可能性，即IRE烧蚀区域主要叠加在电脉冲诱导的焦耳加热热烧蚀区域上。这项研究表明，对于癌症的IRE消融，存在一个真实而实质性的电参数域，它不同于热域，它导致完全的癌细胞消融。实验表明，在三组脉冲中施加1500 V/cm，每组10个脉冲，每个脉冲300微秒，就可以产生完全的癌细胞消融。我们还发现，对于癌细胞消融，使用多个脉冲似乎比在一个脉冲中应用相同的能量更有效。
In this study we perform in vitro irreversible electroporation (IRE) experiments with human hepatocarcinoma cells (HepG2) to investigate IRE as a new technique for undesirable tissue ablation. Irreversible electroporation (IRE) is the irreversible permeabilization of the cell membrane through the application of microsecond through millisecond electrical pulses. Until now IRE was studied only as an undesirable condition during the use of reversible electroporation in gene therapy and electrochemotherapy. There was a possibility that the IRE ablation domain is mostly superimposed on the electrical pulses induced Joule heating thermal ablation domain. This study demonstrates that there is a real and substantial domain of electrical parameters for IRE ablation of cancer that is distinct from the thermal domain and which results in complete cancer cell ablation. Experiments show that the application of 1500 V/cm in three sets of ten pulses of 300 microseconds each can produce complete cancer cell ablation. We also find that the use of multiple pulses appears to be more effective for cancer cell ablation than the application of the same energy in one single pulse.