PREDICTION OF MAJOR HISTOCOMPATIBILITY COMPLEX BINDING REGIONS OF PROTEIN ANTIGENS BY SEQUENCE PATTERN-ANALYSIS
PREDICTION OF MAJOR HISTOCOMPATIBILITY COMPLEX BINDING REGIONS OF PROTEIN ANTIGENS BY SEQUENCE PATTERN-ANALYSIS
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DOI：
10.1073/pnas.86.9.3296
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发表时间：
1989-05-01
影响因子：
11.1
通讯作者：
GREY, HM
中科院分区：
文献类型：
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作者：
SETTE, A;BUUS, S;GREY, HM
我们之前已经实验分析了多肽抗原与d单倍型小鼠主要组织相容性复合体(MHC)分子相互作用的结构要求。我们在这里描述了两个程序，专门用来预测多肽分子与这些MHC II类分子(IAD和IED)相互作用的能力。这些程序的准确性已经在一大组真核、原核和病毒来源的合成肽上进行了测试，还在一组包含整个葡萄球菌核酸酶分子的重叠多肽上进行了测试。对于这两组多肽，在.apprxeq中成功地预测了IAD和IED结合。75%的案例。这表明，这种序列“基序”的定义可以普遍用于预测蛋白质中潜在的免疫原性多肽区域。
We have previously experimentally analyzed the structural requirements for interaction between peptide antigens and mouse major histocompatibility complex (MHC) molecules of the d haplotype. We describe here two procedures devised to predict specifically the capacity of peptide molecules to interact with these MHC class II molecules (IAd and IEd). The accuracy of these procedures has been tested on a large panel of synthetic peptides of eukaryotic, prokaryotic, and viral origin, and also on a set of overlapping peptides encompassing the entire staphylococcal nuclease molecule. For both sets of peptides, IAd and IEd binding was successfully predicted in .apprxeq. 75% of the cases. This suggests that definition of such sequence "motifs" could be of general use in predicting potentially immunogenic peptide regions within proteins.