Identification of WOX Family Genes in Selaginella kraussiana for Studies on Stem Cells and Regeneration in Lycophytes.
Identification of WOX Family Genes in Selaginella kraussiana for Studies on Stem Cells and Regeneration in Lycophytes.
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DOI：
10.3389/fpls.2016.00093
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发表时间：
2016
影响因子：
5.6
通讯作者：
Xu L
中科院分区：
文献类型：
--
作者：
Ge Y;Liu J;Zeng M;He J;Qin P;Huang H;Xu L
植物干细胞产生所有的组织和器官，也是植物再生的来源。在维管植物的进化过程中，植物干细胞的组织结构经历了由简单到复杂的渐进变化。关于植物干细胞的研究大多集中在模式被子植物上，这是维管植物的最新分支分支。然而，我们对其他维管植物中干细胞功能的了解是有限的。石松植物和真叶植物（蕨类植物、裸子植物和被子植物）是维管植物的两个现存分支，它们在4亿多年前分离。石松植物保留了早期维管植物的许多特征。基于基因组和转录组数据，我们确定了WUSCHEL相关HOMEOBOX（WOX）基因在卷柏kraussiana，模式石松，是方便的体外培养和器官形成和再生的观察。WOX基因是控制植物干细胞的关键因素。结果表明，S. kraussiana基因组编码WOX家族的至少8个成员，这代表了WOX家族进化的早期阶段。WOX基因在S. kraussiana为石松干细胞功能的分子研究提供了有用的工具。
Plant stem cells give rise to all tissues and organs and also serve as the source for plant regeneration. The organization of plant stem cells has undergone a progressive change from simple to complex during the evolution of vascular plants. Most studies on plant stem cells have focused on model angiosperms, the most recently diverged branch of vascular plants. However, our knowledge of stem cell function in other vascular plants is limited. Lycophytes and euphyllophytes (ferns, gymnosperms, and angiosperms) are two existing branches of vascular plants that separated more than 400 million years ago. Lycophytes retain many of the features of early vascular plants. Based on genome and transcriptome data, we identified WUSCHEL-RELATED HOMEOBOX (WOX) genes in Selaginella kraussiana, a model lycophyte that is convenient for in vitro culture and observations of organ formation and regeneration. WOX genes are key players controlling stem cells in plants. Our results showed that the S. kraussiana genome encodes at least eight members of the WOX family, which represent an early stage of WOX family evolution. Identification of WOX genes in S. kraussiana could be a useful tool for molecular studies on the function of stem cells in lycophytes.