Follicle-stimulating hormone, human chorionic gonadotropin, and prolactin receptors in hamster corpora lutea or dispersed luteal cells during pregnancy.
Follicle-stimulating hormone, human chorionic gonadotropin, and prolactin receptors in hamster corpora lutea or dispersed luteal cells during pregnancy.
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怀孕期间仓鼠黄体或分散黄体细胞中的卵泡刺激素、人绒毛膜促性腺激素和催乳素受体。
DOI：
10.1095/biolreprod52.2.313
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发表时间：
1995
影响因子：
3.6
通讯作者：
Greenwald,GS
中科院分区：
文献类型：
--
作者：
Yuan,W;Wang,XN;Greenwald,GS
在整个妊娠期间，FSH和LH刺激仓鼠黄体细胞体外产生孕酮（P4）。然而，催乳素（PRL）本身仅在第12天增加P4合成;在第4天，FSH+ LH+ PRL诱导最佳P4分泌[Biol Reprod 1994; 51：43-49]。鉴于这些发现，在这项研究中，我们研究了FSH，hCG和PRL受体在仓鼠CL或分散的黄体细胞在怀孕的第4天，第8天和第12天。第4天和第8天仓鼠CL的Scatchard分析显示，未被占据的hCG受体明显多于FSH受体：第4天，FSH结合位点为9.5 fmol/mg蛋白，hCG结合位点为1741 fmol/mg蛋白。此外，hCG的结合亲和力大于FSH：第4天hCG的IK为0.136 nM，FSH为0.308 nM。在第8天观察到相似的差异。将分散的黄体细胞（大细胞+小细胞）与或不与10 ng绵羊FSH、LH和PRL或人重组FSH（r-hFSH）单独或以不同组合孵育24 h。然后洗涤细胞，并与碘化hCG、FSH或PRL孵育4小时，其中含有或不含有100倍过量的未标记激素。在妊娠第4天和第12天，FSH和hCG每200 000个黄体细胞的结合位点数没有明显变化，而PRL结合位点在妊娠第12天显著增加。与PRL或FSH或FSH+ PRL共孵育24 h后，hCG结合位点在第4天和第12天均显著增加; LH单独不增加hCG结合超过对照值，FSH+ LH、PRL+ LH或FSH+ LH+ PRL上调hCG受体也不超过FSH、PRL或FSH+ PRL的作用。和LH受体在妊娠第4天和第12天的表达支持这些激素的显着体外促黄体作用。然而，FSH+ LH+ PRL对P4的最佳体外合成不能用hCG结合位点的协同增加来解释，而最有可能是cAMP的增加[Biol Reprod 1994; 51：472-479]或其他第二信使系统的结果。
In vitro progesterone (P4) production by hamster luteal cells is stimulated throughout pregnancy by FSH and LH. Prolactin (PRL) by itself, however, increases P4 synthesis only on Day 12; on Day 4, FSH+ LH+ PRL induces optimal P4 secretion [Biol Reprod 1994; 51: 43-49]. In light of these findings, in this study we investigated FSH, hCG, and PRL receptors in hamster CL or dispersed luteal cells on Days 4, 8, and 12 of pregnancy. Scatchard analysis of hamster CL on Days 4 and 8 showed considerably more unoccupied hCG receptors than FSH receptors: on Day 4, there was 9.5 fmol/mg protein for FSH binding sites vs. 1741 fmol/mg protein for hCG binding. Moreover, the binding affinity of hCG was greater than for FSH: the Day 4 IK was 0.136 nM for hCG vs. 0.308 for FSH. Similar differences were observed on Day 8. Dispersed luteal cells (large+ small cells) were incubated for 24 h with or without 10 ng of ovine FSH, LH, and PRL or human recombinant FSH (r-hFSH), alone or in different combinations. The cells were then washed and incubated for 4 h with iodinated hCG, FSH, or PRL with or without 100-fold excess of unlabeled hormones. The number of binding sites per 200 000 luteal cells did not change appreciably for FSH and hCG on Days 4 and 12 of pregnancy, whereas PRL binding sites significantly increased on Day 12. Incubation for 24 h with PRL or FSH or FSH+ PRL statistically increased hCG binding sites on Days 4 and 12; LH alone did not increase hCG binding over control values, nor did FSH+ LH, PRL+ LH, or FSH+ LH+ PRL up-regulate hCG receptors beyond the effects of FSH, PRL, or FSH+ PRL.The presence of FSH, PRL, and LH receptors on Days 4 and 12 of pregnancy supports the significant in vitro luteotropic effects of these hormones. However, the optimal in vitro synthesis of P4 by FSH+ LH+ PRL cannot be explained by a synergistic increase in hCG binding sites and most likely results from an increase of cAMP [Biol Reprod 1994; 51: 472-479] or other second messenger systems.