GENERATION OF A LARGE COMBINATORIAL LIBRARY OF THE IMMUNOGLOBULIN REPERTOIRE IN PHAGE-LAMBDA
GENERATION OF A LARGE COMBINATORIAL LIBRARY OF THE IMMUNOGLOBULIN REPERTOIRE IN PHAGE-LAMBDA
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DOI：
10.1126/science.2531466
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发表时间：
1989-12-08
期刊：
影响因子：
56.9
通讯作者：
LERNER, RA
中科院分区：
文献类型：
--
作者：
HUSE, WD;SASTRY, L;LERNER, RA
利用新型噬菌体Lambda载体系统在大肠杆菌中表达小鼠抗体库中的Fab片段。该系统允许以适合于基因操作的形式快速和容易地鉴定单克隆性Fab片段。有可能在2周内产生大量针对过渡态类似半抗原的单克隆Fab片段。所描述的方法可能取代目前的杂交瘤技术，并促进催化抗体和其他抗体的生产。
A novel bacteriophage lambda vector system was used to express inEscherichia colia combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.