Bovine herpesvirus 5 encodes a unique pattern of microRNAs compared with bovine herpesvirus 1
Bovine herpesvirus 5 encodes a unique pattern of microRNAs compared with bovine herpesvirus 1
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DOI：
10.1099/vir.0.061093-0
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发表时间：
2014-03-01
影响因子：
3.8
通讯作者：
Liu, Zheng-Fei
中科院分区：
文献类型：
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作者：
Tang, Qi;Wu, Yi-Quan;Liu, Zheng-Fei
牛疱疹病毒5型（B 0 HV-5）和牛疱疹病毒1型（BoHV-1）是两种密切相关的病毒。然而，BoHV-5导致小牛的致命性脑膜炎，而BoHV-1与牛的传染性鼻气管炎有关，这两种病毒引起不同症状的机制尚不清楚。在这项研究中，我们确定了11个microRNA（miRNA）基因，由BoHV-5基因组编码，通过深度测序确定，这些基因在生产性感染中被加工成16个可检测的成熟miRNA。我们发现，16个miRNA基因中有6个在内部重复和末端重复区域以两个拷贝存在，导致总共17个miRNA编码位点分布在两条DNA链中。令人惊讶的是，BoHV-5与BoHV-1仅共享一个保守的miRNA，其位于复制起点的上游。此外，与BoHV-1相反，在牛感染细胞蛋白0和潜伏期相关基因内或附近的独特短区域和位点中未检测到miRNA。在参考序列的5'和3'端都观察到变异，导致每个miRNA有一种以上的同种型。茎环逆转录酶-PCR检测16种miRNAs。对具有高读段数的miRNA进行北方印迹分析，检测到所有前体miRNA和一种成熟miRNA。总的来说，这些数据表明，BoHV-5编码不同模式的miRNA，这可能会调节BoHV-5的生命周期，并可能解释与BoHV-1相比，这种病毒的不同发病机制。
Bovine herpesvirus type 5 (B0HV-5) and bovine herpesvirus 1 (BoHV-1) are two closely related viruses. However, BoHV-5 is responsible for fatal meningitis in calves, while BoHV-1 is associated with infectious rhinotracheitis in cattle, and the mechanism by which the two viruses cause different symptoms is not well understood. In this study, we identified 11 microRNA (miRNA) genes, encoded by the BoHV-5 genome, that were processed into 16 detectable mature miRNAs in productive infection as determined by deep sequencing. We found that 6 out of 16 miRNA genes were present as two copies in the internal repeat and terminal repeat regions, resulting in a total of 17 miRNA-encoding loci distributed in both DNA strands. Surprisingly, BoHV-5 shared only one conservative miRNA with BoHV-1, which was located upstream of the origin of replication. Furthermore, in contrast to BoHV-1, no miRNAs were detected in the unique short region and locus within or near the bovine infected-cell protein 0 and latency-related genes. Variations in both the 5' and 3' ends of the reference sequence were observed, resulting in more than one isoform for each miRNA. All of the 16 miRNAs were detectable by stem loop reverse transcriptase-PCR. The miRNAs with high read numbers were subjected to Northern blot analysis, and all pre-miRNAs and one mature miRNA were detected. Collectively, the data suggest that BoHV-5 encodes a different pattern of miRNAs, which may regulate the life cycle of BoHV-5 and might account for the different pathogenesis of this virus compared with BoHV-1.