Highly Sensitive Detection of Organic Molecules on the Basis of a Poly(N-isopropylacrylamide) Microassembly Formed by Plasmonic Optical Trapping
Highly Sensitive Detection of Organic Molecules on the Basis of a Poly(N-isopropylacrylamide) Microassembly Formed by Plasmonic Optical Trapping
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DOI：
10.1021/acs.analchem.6b04024
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发表时间：
2017-01-03
影响因子：
7.4
通讯作者：
Tsuboi, Yasuyuki
中科院分区：
文献类型：
--
作者：
Shoji, Tatsuya;Sugo, Daiki;Tsuboi, Yasuyuki
我们证明，聚（N-异丙基丙烯酰胺）（PNIPAM）微组装，形成等离子体光学捕获，可以提供一个高灵敏度的检测技术的荧光和非荧光有机分子溶解在水溶液中的平台。PNIPAM微组装体可以通过光热效应和增强的光学力的组合而容易地形成。这些物理现象是通过局域表面等离子体激元（LSP）的共振激发获得的。溶解在溶液中的稀疏分布的荧光或非荧光分子可以被提取到PNIPAM组件中，导致荧光或拉曼信号的增加。特别是，我们成功地检测到相当少量的分析物（罗丹明B）在10（-9）mol/L的水平。使用LSP是分析化学中的替代方法，并且除了表面增强拉曼散射和表面增强荧光之外还可以使用。
We demonstrate that a poly(N-isopropylacrylamide) (PNIPAM) microassembly, formed by plasmonic optical trapping, can provide the platform for a highly sensitive detection technique for fluorescent and nonfluorescent organic molecules dissolved in aqueous solution. PNIPAM microassemblies can be easily formed by a combination with a photothermal effect and an enhanced optical force. These physical phenomena were obtained through resonant excitation of localized surface plasmon (LSP). Sparsely distributed fluorescent or nonfluorescent molecules dissolved in solution can be extracted into the PNIPAM assembly, resulting in an increase in fluorescence or Raman signals. In particular, we successfully detected quite small amounts of analytes (rhodamine B) at the 10(-9) mol/L level. Using LSP is an alternative approach in analytical chemistry and can be used in addition to surface enhanced Raman scattering and surface enhanced fluorescence.