A highly reusable MANAE-agarose-immobilized Pleurotus ostreatus laccase for degradation of bisphenol A
A highly reusable MANAE-agarose-immobilized Pleurotus ostreatus laccase for degradation of bisphenol A
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DOI：
10.1016/j.scitotenv.2018.04.051
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发表时间：
2018-09-01
影响因子：
9.8
通讯作者：
Peralta, Rosane Marina
中科院分区：
文献类型：
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作者：
Brugnari, Tatiane;Pereira, Marita Gimenez;Peralta, Rosane Marina
双酚 A (BPA) 是一种内分泌干扰物化合物，持续释放到环境中，并且在废水处理厂中几乎不会降解。先前的一项研究表明，游离平菇漆酶可有效降解 BPA，产生毒性较小的代谢物。在本研究中，该漆酶成功固定在 MANAE-琼脂糖上，提高了其降解 BPA 的效率及其热稳定性和储存稳定性。除此之外，固定化酶在重复使用 15 个周期后仍保留了 90% 以上的初始降解 BPA 的能力。固定在 MANAE-琼脂糖上的 P. ostreatus 漆酶可能是水系统中大规模降解 BPA 的经济替代方案。 (C) 2018 Elsevier B.V.保留所有权利。
Bisphenol A (BPA) is an endocrine disruptor compound that is continuously released into the environment and is barely degraded in wastewater treatment plants. A previous study showed that free Pleurotus ostreatus laccase is efficient in degrading BPA producing less toxic metabolites. In the present study, this laccase was successfully immobilized onto MANAE-agarose, improving its efficiency in degrading BPA and its thermal and storage stabilities. In addition to this, the immobilized enzyme retained >90% of its initial capability to degrade BPA after 15 cycles of reuse. P. ostreatus laccase immobilized onto MANAE-agarose could be an economical alternative for large scale degradation of BPA in aqueous systems. (C) 2018 Elsevier B. V.All rights reserved.