CsWRKY10 mediates defence responses to Botrytis cinerea infection in Cucumis sativus
CsWRKY10 mediates defence responses to Botrytis cinerea infection in Cucumis sativus
复制标题
CsWRKY10 介导黄瓜对灰葡萄孢感染的防御反应
DOI：
10.1016/j.plantsci.2020.110640
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发表时间：
2020
期刊：
影响因子：
5.2
通讯作者：
Ren Zhonghai
中科院分区：
文献类型：
--
作者：
Liu Mengyu;Zhang Qingxia;Wang Can;Meng Tianqi;Wang Lina;Chen Chunhua;Ren Zhonghai
黄瓜（Cucumis sativus）是世界上种植最广泛的蔬菜作物之一，灰葡萄孢（B.灰霉病），导致严重疾病。然而，很少有基因参与对B.在黄瓜中发现了灰霉病菌。在本研究中，我们发现CsWRKY10在黄瓜抗B. CsWRKY10的过表达增加了B.黄瓜病原菌侵染后，转基因植株体内过氧化氢酶、超氧化物歧化酶和过氧化物酶的活性受到影响，导致活性氧（ROS）含量下降。此外，光学显微镜观察表明，CsWRKY10的过表达促进了B.黄瓜重要的是，B。结果表明，转基因植株中茉莉酸（JA）含量降低，JA和水杨酸相关防御基因的表达水平显著改变。相反，CsWRKY10的过表达增强了黄瓜对玉米棒孢霉的抗性。综上所述，本研究表明CsWRKY10负调控黄瓜对B.灰霉病能降低活性氧含量，抑制茉莉酸介导的抗性信号通路，但增强了对玉米棒孢霉的抗性。
Cucumber (Cucumis sativus) is one of the most widely cultivated vegetable crops in the world, and its yield is often reduced due to the infection ofBotrytis cinerea(B. cinerea), which causes a serious disease. However, few genes involved in the response toB. cinereahave been identified in cucumber. In this study, we identified thatCsWRKY10plays a key role in the cucumber resistance toB. cinereabecause that the overexpression ofCsWRKY10significantly increased the susceptibility toB. cinereain cucumber. After the pathogen infection, the enzyme activities of catalase, superoxide dismutase and peroxidase in transgenic plants were affected, resulting in the decrease in reactive oxygen species (ROS) contents. In addition, the light microscopic images showed that overexpression ofCsWRKY10promoted the spore germination and mycelia elongation ofB. cinereain cucumber. Importantly, afterB. cinereainfection, the contents of jasmonic acid (JA) are decreased, and the expression levels of JA- and salicylic acid- related defence genes significantly changed in transgenic plants. In contrast, overexpression ofCsWRKY10enhanced resistance toCorynespora cassiicolain cucumber. Collectively, this study indicated thatCsWRKY10negatively regulates the resistance of cucumber toB. cinereaby reducing the ROS contents and inhibiting the JA-mediated resistance signalling pathway, but strengthens resistance toCorynespora cassiicola.