Gonadotropin-releasing hormone-dependent regulation of gonadotropin subunit messenger ribonucleic acid levels in the rat.
Gonadotropin-releasing hormone-dependent regulation of gonadotropin subunit messenger ribonucleic acid levels in the rat.
复制标题
大鼠促性腺激素释放激素依赖性调节促性腺激素亚基信使核糖核酸水平。
作者：
M. Wierman;J. Rivier;Chun Wang
在性腺切除术后7天，对去势雄性和雌性大鼠给予Nal-Glu GnRH拮抗剂（GnRHA），以评估选择性GnRH抑制对FSH β、LH β和α亚基稳态mRNA水平以及FSH和LH血清水平的影响。对雌性大鼠给予低剂量GnRHA（125 μ g/kg.day）1、3或7天，导致血清FSH和LH水平抑制7天，分别为卵巢切除对照值的50%和40%。LH β mRNA水平以时间依赖性方式降低，因此到第7天，LH β mRNA水平低于完整对照组。α和FSH β mRNA水平有显著但不太显著的下降。对去势雄性或雌性大鼠给予更高剂量的GnRHA（500 μ g/kg.day）7或14天，导致血清LH和FSH抑制至或低于完整对照组的水平。在这个剂量下，所有三种促性腺激素亚基mRNA水平从去势值下降到或低于完整对照组。因此，尽管低剂量GnRHA给药对LH β mRNA的抑制作用大于FSH β mRNA水平，但高剂量GnRHA给药对所有三种促性腺激素亚基的抑制作用相同。两种剂量的GnRHA均未观察到对α亚基mRNA水平的刺激作用。我们的结论是促性腺激素亚基生物合成的翻译前控制是GnRH依赖的。强效Nal-Glu GnRHA的适当剂量和给药时间可抑制雌性和雄性大鼠的血清促性腺激素FSH和LH以及FSH β、LH β和α亚基的mRNA。
The Nal-Glu GnRH antagonist (GnRHA) was given to castrate male and female rats 7 days after gonadectomy to assess the impact of selective GnRH inhibition on the steady state mRNA levels of FSH beta, LH beta, and alpha-subunit and serum levels of FSH and LH. A low dose of GnRHA (125 micrograms/kg.day) given to female rats for 1, 3, or 7 days resulted in suppression of serum FSH and LH levels by 7 days to 50% and 40%, respectively, of ovariectomized control values. LH beta mRNA levels decreased in a time-dependent manner, so that by 7 days, LH beta mRNA levels were less than those in intact controls. There were significant but less dramatic declines in alpha and FSH beta mRNA levels. A higher dose of GnRHA (500 micrograms/kg.day) for 7 or 14 days administered to castrate male or female rats resulted in inhibition of serum LH and FSH to or below levels in intact controls. At this dose, all three gonadotropin subunit mRNA levels fell from castrate values toward or below those in intact controls. Thus, although low dose GnRHA administration suppressed LH beta mRNA more than FSH beta mRNA levels, high dose GnRHA treatment resulted in equal suppression of all three gonadotropin subunits. No stimulatory effects on alpha-subunit mRNA levels were observed with either dose of GnRHA. We conclude that the pretranslational control of gonadotropin subunit biosynthesis is GnRH dependent. Adequate dose and length of administration of the potent Nal-Glu GnRHA results in suppression of both the serum gonadotropins FSH and LH and the mRNAs for FSH beta, LH beta, and alpha-subunit in female and male rats.
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DOI：
10.1210/jcem-58-6-1050
发表时间：
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期刊：
The Journal of clinical endocrinology and metabolism
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DOI：
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发表时间：
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DOI：
10.1016/b978-0-12-571141-8.50015-9
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期刊：
Recent progress in hormone research
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--
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影响因子：
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影响因子：
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作者：
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