Expression of AA4.1 marks lymphohematopoietic progenitors in early mouse development.
Expression of AA4.1 marks lymphohematopoietic progenitors in early mouse development.
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AA4.1 的表达标志着小鼠早期发育中的淋巴造血祖细胞。
DOI：
10.1073/pnas.0904090106
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发表时间：
2009
影响因子：
11.1
通讯作者：
Weissman,IrvingL
中科院分区：
文献类型：
--
作者：
Yamane,Toshiyuki;Hosen,Naoki;Yamazaki,Hidetoshi;Weissman,IrvingL
小鼠的造血系统是在发育的早期到中期建立起来的。然而，与胎肝和骨髓的造血干细胞室相比，在小鼠发育过程中出现的最早的淋巴造血祖细胞的特性还不够好。我们用胚胎干细胞体外培养的方法分离出了最早的淋巴造血祖细胞。与基质细胞共培养的ES细胞中存在大量具有c-Kit+lin−细胞表型的细胞。根据AA4.1的表达，我们进一步将细胞分为两个不同的细胞亚集。尽管AA4.1+和AA4.1−细胞具有相同的生成髓系细胞系的能力，但ES细胞来源的淋巴潜能在很大程度上限于表达AA4.1的细胞。同样的细胞类型在早期卵黄囊中大量存在，而在发育中的尾部胚胎中数量较少(≈为卵黄囊中的5%)。这些数据表明，AA4.1是一种细胞表面标记，可以识别小鼠发育中最早的淋巴造血祖细胞。
The hematopoietic system of mice is established during the early to midgestational stage of development. However, the earliest lymphohematopoietic progenitors that appear during mouse development have been less well characterized compared with the hematopoietic stem cell compartment of fetal liver and bone marrow. We isolated the earliest lymphohematopoietic progenitors by using embryonic stem (ES) cell culture in vitro. Cells with the c-Kit+Lin−cell surface phenotype were present abundantly in ES cells cocultured with stromal cell lines. We further separated the cells into two distinct cell subsets based on AA4.1 expression. Although AA4.1+and AA4.1−cells had equivalent potency to generate myeloid cell lineages, the lymphoid potential in ES-cell-derived cells was largely restricted to the cells expressing AA4.1. The same cell type was present abundantly in the early yolk sac and in fewer numbers (≈5% of that in the yolk sac) in the caudal half of the developing embryos. These data suggest that AA4.1 is a cell surface marker that can identify the earliest lymphohematopoietic progenitors in mouse development.