Linkage of microbial ecology to phenotype: correlation of rumen microbial ecology to cattle's feed efficiency
Linkage of microbial ecology to phenotype: correlation of rumen microbial ecology to cattle's feed efficiency
复制标题
DOI：
10.1111/j.1574-6968.2008.01343.x
复制
发表时间：
2008-11-01
影响因子：
2.1
通讯作者：
Moore, Stephen S.
中科院分区：
文献类型：
--
作者：
Guan, Le Luo;Nkrumah, Joshua D.;Moore, Stephen S.
瘤胃微生物结构与宿主表型性状的联系可能会增强对家畜物种中宿主-微生物相互作用的理解。本研究采用不依赖培养的PCR-变性梯度凝胶电泳（PCR-DGGE）技术，对不同饲料效率牛瘤胃微生物组成进行了研究。可检测的细菌PCR-DGGE图谱的分析表明，从高效肉牛产生的图谱聚集在一起，并从低效肉牛中获得的图谱中清楚地分离出来，表明特定的细菌群可能只栖息在高效肉牛中。此外，细菌谱更可能聚集在某一品种内，表明宿主遗传可能在瘤胃微生物结构中起重要作用。挥发性脂肪酸含量与饲料效率性状之间存在相关性。高效牛的丁酸（P < 0.001）和戊酸（P=0.006）浓度显著高于对照牛。我们的研究结果揭示了可检测的瘤胃微生物群及其发酵参数与牛饲料效率之间的潜在关联。
Linkage of rumen microbial structure to host phenotypical traits may enhance the understanding of host-microbial interactions in livestock species. This study used culture-independent PCR-denaturing gradient gel electrophoresis (PCR-DGGE) to investigate the microbial profiles in the rumen of cattle differing in feed efficiency. The analysis of detectable bacterial PCR-DGGE profiles showed that the profiles generated from efficient steers clustered together and were clearly separated from those obtained from inefficient steers, indicating that specific bacterial groups may only inhabit in efficient steers. In addition, the bacterial profiles were more likely clustered within a certain breed, suggesting that host genetics may play an important role in rumen microbial structure. The correlations between the concentrations of volatile fatty acids and feed efficiency traits were also observed. Significantly higher concentrations of butyrate (P < 0.001) and valerate (P=0.006) were detected in the efficient steers. Our results revealed potential associations between the detectable rumen microbiota and its fermentation parameters with the feed efficiency of cattle.