A fluorescent probe for cysteine depalmitoylation reveals dynamic APT signaling.
A fluorescent probe for cysteine depalmitoylation reveals dynamic APT signaling.
复制标题
半胱氨酸二光二酰化的荧光探针揭示了动态的APT信号传导。
DOI：
10.1038/nchembio.2262
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发表时间：
2017-02
影响因子：
14.8
通讯作者：
Dickinson BC
中科院分区：
文献类型：
--
作者：
Kathayat RS;Elvira PD;Dickinson BC
数百种人类蛋白质通过半胱氨酸残基的可逆棕榈酰化（S-棕榈酰化）进行修饰，但对脱棕榈酰化的调控知之甚少。在这里，我们开发了“脱棕榈酰化探针”（DPP），小分子荧光团，以监测内源性活性水平的“橡皮擦”的S-棕榈酰化，酰基蛋白硫酯酶（APT）。DPP的活细胞分析揭示了快速生长因子介导的APTs脱棕榈酰化活性的抑制，揭示了动态脂质信号传导的新调控机制。
Hundreds of human proteins are modified by reversible palmitoylation of cysteine residues (S-palmitoylation), but the regulation of depalmitoylation is poorly understood. Here, we develop “depalmitoylation probes” (DPPs), small molecule fluorophores to monitor the endogenous activity levels of “erasers” of S-palmitoylation, acyl-protein thioesterases (APTs). Live-cell analysis with DPPs reveals rapid growth factor-mediated inhibition of the depalmitoylation activity of APTs, exposing a novel regulatory mechanism of dynamic lipid signaling.