Engineering Stem Cell Organoids.
Engineering Stem Cell Organoids.
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DOI：
10.1016/j.stem.2015.12.005
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发表时间：
2016-01-07
期刊：
影响因子：
23.9
通讯作者：
Levy O
中科院分区：
文献类型：
--
作者：
Yin X;Mead BE;Safaee H;Langer R;Karp JM;Levy O
类器官系统利用干细胞的自组织特性来创建多样化的多细胞组织代理。大多数类器官模型仅代表组织的单个或部分组分，并且通常难以控制这些系统中的细胞类型、组织和细胞-细胞/细胞-基质相互作用。在本文中，我们讨论了生成基于干细胞的类器官的基本方法，它们的优点和局限性，以及如何使用生物工程策略来控制类器官内的细胞组成及其3D组织，以进一步增强它们在研究和治疗中的效用。
Organoid systems leverage the self-organizing properties of stem cells to create diverse multi-cellular tissue proxies. Most organoid models only represent single or partial components of a tissue, and it is often difficult to control the cell type, organization, and cell-cell/cell-matrix interactions within these systems. Herein, we discuss basic approaches to generate stem cell-based organoids, their advantages and limitations, and how bioengineering strategies can be used to steer the cell composition and their 3D organization within organoids to further enhance their utility in research and therapies.